Abstract The mitigation of AMD at Canadian mine sites using passive treatment technologies requires a combined strategy that minimizes the effect of climatic variability on the treatment performance of the system. A vertical-flow combined passive treatment system was developed consisting of an oxidation/precipitation basin for excess iron removal; an unsaturated flow peat biofilter for heavy metal sorption and the reduction of oxygen concentrations; a sulfate reducing bacteria bioreactor for alkalinity generation and sulfate reduction; and, an anoxic limestone drain for alkalinity addition. Laboratory investigations were undertaken to characterize AMD mitigation through the peat biofilter under unsaturated flow conditions and to assess its performance in the combined passive system. Peat column sorption studies were conducted at 20 and 0°C to assess the effect of temperature on metal retention. At 0°C, a metal removal efficiency of Cd < Mn < Zn < Al < Ni < Cu < Fe was noted. At 20°C, however, sulfate-reducing conditions developed in the column as a result of the flow regime, which facilitated the establishment of anaerobic zones within the peat column, leading to a significant increase in effluent pH and metal removal. Sequential extraction indicated that Fe, Cu, and Al were typically bound to the organic matter fraction, while Mn, Zn, Ni, and Cd were primarily associated with the exchangeable fraction.