An Overview of the Analysis of Next Generation Sequencing Data
edited by: Junbai Wang, Aik C. Tan, Tianhai Tian
Next generation sequencing is a common and versatile tool for biological and medical research. We describe the basic steps for analyzing next generation sequencing data, including quality checking and mapping to a reference genome. We also explain the further data analysis for three common applications of next generation sequencing: variant detection, RNA-seq, and ChIP-seq.