Characteristic defects in neural crest cell-specific G[alpha]q/G[alpha]11- and G[alpha]12/G[alpha]13-deficient mice Export

@article{citeulike:1314976, abstract = {The endothelin/endothelin receptor system plays a critical role in the differentiation and terminal migration of particular neural crest cell subpopulations. Targeted deletion of the G-protein-coupled endothelin receptors ETA and ETB was shown to result in characteristic developmental defects of derivatives of cephalic and cardiac neural crest and of neural crest-derived melanocytes and enteric neurons, respectively. Since both endothelin receptors are coupled to G-proteins of the Gq/G11- and G12/G13-families, we generated mouse lines lacking G[alpha]q/G[alpha]11 or G[alpha]12/G[alpha]13 in neural crest cells to study their roles in neural crest development. Mice lacking G[alpha]q/G[alpha]11 in a neural crest cell-specific manner had craniofacial defects similar to those observed in mice lacking the ETA receptor or endothelin-1 (ET-1). However, in contrast to ET-1/ETA mutant animals, cardiac outflow tract morphology was intact. Surprisingly, neither G[alpha]q/G[alpha]11- nor G[alpha]12/G[alpha]13-deficient mice showed developmental defects seen in animals lacking either the ETB receptor or its ligand endothelin-3 (ET-3). Interestingly, G[alpha]12/G[alpha]13 deficiency in neural crest cell-derived cardiac cells resulted in characteristic cardiac malformations. Our data show that Gq/G11- but not G12/G13-mediated signaling processes mediate ET-1/ETA-dependent development of the cephalic neural crest. In contrast, ET-3/ETB-mediated development of neural crest-derived melanocytes and enteric neurons appears to involve G-proteins different from Gq/G11/G12/G13.}, author = {Dettlaff-Swiercz, Dagmara A. and Wettschureck, Nina and Moers, Alexandra and Huber, Katrin and Offermanns, Stefan}, citeulike-article-id = {1314976}, citeulike-linkout-0 = {http://www.sciencedirect.com/science/article/B6WDG-4FW7MBM-3/2/69d358bda211dc3217bed01753cd754f}, day = {1}, journal = {Developmental Biology}, keywords = {g12, g13}, month = {June}, number = {1}, pages = {174--182}, posted-at = {2007-05-21 14:01:32}, priority = {2}, title = {Characteristic defects in neural crest cell-specific G[alpha]q/G[alpha]11- and G[alpha]12/G[alpha]13-deficient mice}, url = {http://www.sciencedirect.com/science/article/B6WDG-4FW7MBM-3/2/69d358bda211dc3217bed01753cd754f}, volume = {282}, year = {2005} }

TY - JOUR ID - citeulike:1314976 L3 - citeulike-article-id:1314976 N2 - The endothelin/endothelin receptor system plays a critical role in the differentiation and terminal migration of particular neural crest cell subpopulations. Targeted deletion of the G-protein-coupled endothelin receptors ETA and ETB was shown to result in characteristic developmental defects of derivatives of cephalic and cardiac neural crest and of neural crest-derived melanocytes and enteric neurons, respectively. Since both endothelin receptors are coupled to G-proteins of the Gq/G11- and G12/G13-families, we generated mouse lines lacking G[alpha]q/G[alpha]11 or G[alpha]12/G[alpha]13 in neural crest cells to study their roles in neural crest development. Mice lacking G[alpha]q/G[alpha]11 in a neural crest cell-specific manner had craniofacial defects similar to those observed in mice lacking the ETA receptor or endothelin-1 (ET-1). However, in contrast to ET-1/ETA mutant animals, cardiac outflow tract morphology was intact. Surprisingly, neither G[alpha]q/G[alpha]11- nor G[alpha]12/G[alpha]13-deficient mice showed developmental defects seen in animals lacking either the ETB receptor or its ligand endothelin-3 (ET-3). Interestingly, G[alpha]12/G[alpha]13 deficiency in neural crest cell-derived cardiac cells resulted in characteristic cardiac malformations. Our data show that Gq/G11- but not G12/G13-mediated signaling processes mediate ET-1/ETA-dependent development of the cephalic neural crest. In contrast, ET-3/ETB-mediated development of neural crest-derived melanocytes and enteric neurons appears to involve G-proteins different from Gq/G11/G12/G13. IS - 1 JF - Developmental Biology EP - 182 TI - Characteristic defects in neural crest cell-specific G[alpha]q/G[alpha]11- and G[alpha]12/G[alpha]13-deficient mice VL - 282 SP - 174 KW - g12 KW - g13 AU - Dettlaff-Swiercz, Dagmara AU - Wettschureck, Nina AU - Moers, Alexandra AU - Huber, Katrin AU - Offermanns, Stefan PY - 2005/06/01/ UR - http://www.sciencedirect.com/science/article/B6WDG-4FW7MBM-3/2/69d358bda211dc3217bed01753cd754f ER -