Serotonergic regulation of membrane potential in developing rat prefrontal cortex: coordinated expression of 5-hydroxytryptamine (5-HT)1A, 5-HT2A, and 5-HT7 receptors. Export

@article{citeulike:3736447, abstract = {The developing prefrontal cortex receives a dense serotonergic innervation, yet little is known about the actions of serotonin [5-Hydroxytryptamine (5-HT)] in this region during development. Here, we examined the developmental regulation of 5-HT receptors controlling the excitability of pyramidal neurons of this region. Using whole-cell recordings in in vitro brain slices, we identified a dramatic shift in the effects of 5-HT on membrane potential during the postnatal developmental period. In slices derived from young animals [postnatal day (P) 6 to P19], administration of 5-HT elicits a robust depolarization of layer V pyramidal neurons, which gradually shifts to a hyperpolarization commencing during the third postnatal week. This progression is the result of coordinated changes in the function of 5-HT7 and 5-HT2A receptors, which mediate different aspects of the depolarization, and of 5-HT1A receptors, which signal the late developing hyperpolarization. The loss of the 5-HT7 receptor-mediated depolarization and the appearance of the 5-HT1A receptor-mediated hyperpolarization appears to reflect changes in receptor expression. In contrast, the decline in the 5-HT2A receptor depolarization with increasing age was associated with changes in the effectiveness with which these receptors could elicit a membrane depolarization, rather than loss of the receptors per se. Together, these results outline coordinated changes in the serotonergic regulation of cortical excitability at a time of extensive synaptic development and thus suggest a key role for these receptor subtypes in the postnatal development of the prefrontal cortex.}, author = {B\'{e}\"{i}que, J. C. and Campbell, B. and Perring, P. and Hamblin, M. W. and Walker, P. and Mladenovic, L. and Andrade, R.}, citeulike-article-id = {3736447}, citeulike-linkout-0 = {http://dx.doi.org/10.1523/JNEUROSCI.5113-03.2004}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15152041}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15152041}, day = {19}, doi = {10.1523/JNEUROSCI.5113-03.2004}, issn = {1529-2401}, journal = {The Journal of neuroscience : the official journal of the Society for Neuroscience}, keywords = {5-ht1, 5-ht7, serotonin}, month = {May}, number = {20}, pages = {4807--4817}, posted-at = {2008-12-02 17:13:40}, priority = {2}, title = {Serotonergic regulation of membrane potential in developing rat prefrontal cortex: coordinated expression of 5-hydroxytryptamine (5-HT)1A, 5-HT2A, and 5-HT7 receptors.}, url = {http://dx.doi.org/10.1523/JNEUROSCI.5113-03.2004}, volume = {24}, year = {2004} }

TY - JOUR ID - citeulike:3736447 L3 - citeulike-article-id:3736447 N2 - The developing prefrontal cortex receives a dense serotonergic innervation, yet little is known about the actions of serotonin [5-Hydroxytryptamine (5-HT)] in this region during development. Here, we examined the developmental regulation of 5-HT receptors controlling the excitability of pyramidal neurons of this region. Using whole-cell recordings in in vitro brain slices, we identified a dramatic shift in the effects of 5-HT on membrane potential during the postnatal developmental period. In slices derived from young animals [postnatal day (P) 6 to P19], administration of 5-HT elicits a robust depolarization of layer V pyramidal neurons, which gradually shifts to a hyperpolarization commencing during the third postnatal week. This progression is the result of coordinated changes in the function of 5-HT7 and 5-HT2A receptors, which mediate different aspects of the depolarization, and of 5-HT1A receptors, which signal the late developing hyperpolarization. The loss of the 5-HT7 receptor-mediated depolarization and the appearance of the 5-HT1A receptor-mediated hyperpolarization appears to reflect changes in receptor expression. In contrast, the decline in the 5-HT2A receptor depolarization with increasing age was associated with changes in the effectiveness with which these receptors could elicit a membrane depolarization, rather than loss of the receptors per se. Together, these results outline coordinated changes in the serotonergic regulation of cortical excitability at a time of extensive synaptic development and thus suggest a key role for these receptor subtypes in the postnatal development of the prefrontal cortex. IS - 20 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience SN - 1529-2401 EP - 4817 TI - Serotonergic regulation of membrane potential in developing rat prefrontal cortex: coordinated expression of 5-hydroxytryptamine (5-HT)1A, 5-HT2A, and 5-HT7 receptors. VL - 24 SP - 4807 KW - 5-ht1 KW - 5-ht7 KW - serotonin AU - Béïque, JC AU - Campbell, B AU - Perring, P AU - Hamblin, MW AU - Walker, P AU - Mladenovic, L AU - Andrade, R PY - 2004/05/19/ UR - http://dx.doi.org/10.1523/JNEUROSCI.5113-03.2004 ER -