The Fluorescent Toolbox for Assessing Protein Location and Function Export

@article{citeulike:590367, abstract = {Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy. Small organic fluorescent dyes, nanocrystals ("quantum dots"), autofluorescent proteins, small genetic encoded tags that can be complexed with fluorochromes, and combinations of these probes are highlighted. 10.1126/science.1124618}, author = {Giepmans, Ben N. G. and Adams, Stephen R. and Ellisman, Mark H. and Tsien, Roger Y.}, citeulike-article-id = {590367}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1124618}, citeulike-linkout-1 = {http://www.sciencemag.org/content/312/5771/217.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/312/5771/217.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/312/5771/217}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/16614209}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=16614209}, day = {14}, doi = {10.1126/science.1124618}, journal = {Science}, keywords = {fluorescence-dyes, fluorescent\_proteins, review}, month = {April}, number = {5771}, pages = {217--224}, posted-at = {2006-07-18 22:40:40}, priority = {2}, title = {The Fluorescent Toolbox for Assessing Protein Location and Function}, url = {http://dx.doi.org/10.1126/science.1124618}, volume = {312}, year = {2006} }

TY - JOUR ID - citeulike:590367 L3 - citeulike-article-id:590367 N2 - Advances in molecular biology, organic chemistry, and materials science have recently created several new classes of fluorescent probes for imaging in cell biology. Here we review the characteristic benefits and limitations of fluorescent probes to study proteins. The focus is on protein detection in live versus fixed cells: determination of protein expression, localization, activity state, and the possibility for combination of fluorescent light microscopy with electron microscopy. Small organic fluorescent dyes, nanocrystals ("quantum dots"), autofluorescent proteins, small genetic encoded tags that can be complexed with fluorochromes, and combinations of these probes are highlighted. 10.1126/science.1124618 IS - 5771 JF - Science EP - 224 TI - The Fluorescent Toolbox for Assessing Protein Location and Function VL - 312 SP - 217 KW - fluorescence-dyes KW - fluorescent_proteins KW - review AU - Giepmans, Ben AU - Adams, Stephen AU - Ellisman, Mark AU - Tsien, Roger PY - 2006/04/14/ UR - http://dx.doi.org/10.1126/science.1124618 ER -