Fluorescence lifetime imaging microscopy (FLIM). Export

@article{citeulike:895492, abstract = {Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems have been implemented both in the frequency domain, using sinusoidally intensity-modulated excitation light and modulated detectors, and in the time domain, using pulsed excitation sources and time-correlated or time-gated detection. In this review we describe the different modes in which both frequency-domain and time-domain FLIM instruments have been constructed in wide-field and in point-scanning (confocal) microscopes. Also, novel additional strategies for constructing FLIM-instruments are discussed. In addition to technical implementation, this chapter gives an overview of the application of FLIM in cell biological en biomedical studies. Especially for in situ protein-protein interaction studies using fluorescence resonance energy transfer (FRET), FLIM has proven to be a robust and established technique in modern cell biology. Other application areas, including usage of lifetime contrast for ion-imaging, quantitative imaging, tissue characterization and medical applications, are discussed.}, address = {Swammerdam Institute for Life Sciences \& Centre for Advanced Microscopy, Section Molecular Cytology, Kruislaan 316, 1098 SM Amsterdam, The Netherlands. munster@science.uva.nl}, author = {van Munster, Erik B. and Gadella, Theodorus W.}, citeulike-article-id = {895492}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/16080268}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=16080268}, issn = {0724-6145}, journal = {Advances in biochemical engineering/biotechnology}, keywords = {flim, review}, pages = {143--175}, posted-at = {2006-10-13 16:06:15}, priority = {2}, title = {Fluorescence lifetime imaging microscopy (FLIM).}, url = {http://view.ncbi.nlm.nih.gov/pubmed/16080268}, volume = {95}, year = {2005} }

TY - JOUR ID - citeulike:895492 L3 - citeulike-article-id:895492 N2 - Fluorescence lifetime imaging microscopy (FLIM) is a technique to map the spatial distribution of nanosecond excited state lifetimes within microscopic images. FLIM systems have been implemented both in the frequency domain, using sinusoidally intensity-modulated excitation light and modulated detectors, and in the time domain, using pulsed excitation sources and time-correlated or time-gated detection. In this review we describe the different modes in which both frequency-domain and time-domain FLIM instruments have been constructed in wide-field and in point-scanning (confocal) microscopes. Also, novel additional strategies for constructing FLIM-instruments are discussed. In addition to technical implementation, this chapter gives an overview of the application of FLIM in cell biological en biomedical studies. Especially for in situ protein-protein interaction studies using fluorescence resonance energy transfer (FRET), FLIM has proven to be a robust and established technique in modern cell biology. Other application areas, including usage of lifetime contrast for ion-imaging, quantitative imaging, tissue characterization and medical applications, are discussed. JF - Advances in biochemical engineering/biotechnology SN - 0724-6145 AD - Swammerdam Institute for Life Sciences & Centre for Advanced Microscopy, Section Molecular Cytology, Kruislaan 316, 1098 SM Amsterdam, The Netherlands. munster@science.uva.nl EP - 175 TI - Fluorescence lifetime imaging microscopy (FLIM). VL - 95 SP - 143 KW - flim KW - review AU - van Munster, Erik AU - Gadella, Theodorus PY - 2005/// UR - http://view.ncbi.nlm.nih.gov/pubmed/16080268 ER -