Colonic tumorigenesis in BubR1+/-ApcMin/+ compound mutant mice is linked to premature separation of sister chromatids and enhanced genomic instability. Export

@article{citeulike:3644656, abstract = {Faithful chromosome segregation is essential for the maintenance of genetic stability during cell division and it is at least partly monitored by the spindle checkpoint, a surveillance mechanism preventing the cell from prematurely entering anaphase. The adenomatous polyposis coli (Apc) gene also plays an important role in regulating genomic stability, as mutations of Apc cause aneuploidy. Here we show that whereas Apc(Min)(/+) mice developed many adenomatous polyps, mostly in the small intestine, by 3 mo of age; BubR1(+/-)Apc(Min)(/+) compound mutant mice developed 10 times more colonic tumors than Apc(Min)(/+) mice. The colonic tumors in BubR1(+/-)Apc(Min)(/+) mice were in higher grades than those observed in Apc(Min)(/+) mice. Consistently, BubR1(+/-)Apc(Min)(/+) murine embryonic fibroblasts (MEFs) contained more beta-catenin and proliferated at a faster rate than WT or BubR1(+/-) MEFs. Moreover, BubR1(+/-)Apc(Min)(/+) MEFs slipped through mitosis in the presence of nocodazole and exhibited a higher rate of genomic instability than that of WT or BubR1(+/-) or Apc(Min)(/+) MEFs, accompanied by premature separation of sister chromatids. Together, our studies suggest that BubR1 and Apc functionally interact in regulating metaphase-anaphase transition, deregulation of which may play a key role in genomic instability and development and progression of colorectal cancer.}, author = {Rao, C. V. and Yang, Y. M. and Swamy, M. V. and Liu, T. and Fang, Y. and Mahmood, R. and Jhanwar-Uniyal, M. and Dai, W.}, citeulike-article-id = {3644656}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0407822102}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15767571}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15767571}, day = {22}, doi = {10.1073/pnas.0407822102}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {aneuploidy, cancer, colon, colorectal, genomic, instability}, month = {March}, number = {12}, pages = {4365--4370}, posted-at = {2008-11-21 11:35:44}, priority = {2}, title = {Colonic tumorigenesis in BubR1+/-ApcMin/+ compound mutant mice is linked to premature separation of sister chromatids and enhanced genomic instability.}, url = {http://dx.doi.org/10.1073/pnas.0407822102}, volume = {102}, year = {2005} }

TY - JOUR ID - citeulike:3644656 L3 - citeulike-article-id:3644656 N2 - Faithful chromosome segregation is essential for the maintenance of genetic stability during cell division and it is at least partly monitored by the spindle checkpoint, a surveillance mechanism preventing the cell from prematurely entering anaphase. The adenomatous polyposis coli (Apc) gene also plays an important role in regulating genomic stability, as mutations of Apc cause aneuploidy. Here we show that whereas Apc(Min)(/+) mice developed many adenomatous polyps, mostly in the small intestine, by 3 mo of age; BubR1(+/-)Apc(Min)(/+) compound mutant mice developed 10 times more colonic tumors than Apc(Min)(/+) mice. The colonic tumors in BubR1(+/-)Apc(Min)(/+) mice were in higher grades than those observed in Apc(Min)(/+) mice. Consistently, BubR1(+/-)Apc(Min)(/+) murine embryonic fibroblasts (MEFs) contained more beta-catenin and proliferated at a faster rate than WT or BubR1(+/-) MEFs. Moreover, BubR1(+/-)Apc(Min)(/+) MEFs slipped through mitosis in the presence of nocodazole and exhibited a higher rate of genomic instability than that of WT or BubR1(+/-) or Apc(Min)(/+) MEFs, accompanied by premature separation of sister chromatids. Together, our studies suggest that BubR1 and Apc functionally interact in regulating metaphase-anaphase transition, deregulation of which may play a key role in genomic instability and development and progression of colorectal cancer. IS - 12 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 EP - 4370 TI - Colonic tumorigenesis in BubR1+/-ApcMin/+ compound mutant mice is linked to premature separation of sister chromatids and enhanced genomic instability. VL - 102 SP - 4365 KW - aneuploidy KW - cancer KW - colon KW - colorectal KW - genomic KW - instability AU - Rao, CV AU - Yang, YM AU - Swamy, MV AU - Liu, T AU - Fang, Y AU - Mahmood, R AU - Jhanwar-Uniyal, M AU - Dai, W PY - 2005/03/22/ UR - http://dx.doi.org/10.1073/pnas.0407822102 ER -