ETO-2 Associates with SCL in Erythroid Cells and Megakaryocytes and Provides Repressor Functions in Erythropoiesis Export

@article{schuh_eto-2_2005, abstract = {Lineage specification and cellular maturation require coordinated regulation of gene expression programs. In large part, this is dependent on the activator and repressor functions of protein complexes associated with tissue-specific transcriptional regulators. In this study, we have used a proteomic approach to characterize multiprotein complexes containing the key hematopoietic regulator SCL in erythroid and megakaryocytic cell lines. One of the novel SCL-interacting proteins identified in both cell types is the transcriptional corepressor ETO-2. Interaction between endogenous proteins was confirmed in primary cells. We then showed that SCL complexes are shared but also significantly differ in the two cell types. Importantly, SCL/ETO-2 interacts with another corepressor, Gfi-1b, in red cells but not megakaryocytes. The SCL/ETO-2/Gfi-1b association is lost during erythroid differentiation of primary fetal liver cells. Genetic studies of erythroid cells show that ETO-2 exerts a repressor effect on SCL target genes. We suggest that, through its association with SCL, ETO-2 represses gene expression in the early stages of erythroid differentiation and that alleviation/modulation of the repressive state is then required for expression of genes necessary for terminal erythroid maturation to proceed.}, author = {Schuh, Anna H. and Tipping, Alex J. and Clark, Allison J. and Hamlett, Isla and Guyot, Boris and Iborra, Francesco J. and Rodriguez, Patrick and Strouboulis, John and Enver, Tariq and Vyas, Paresh and Porcher, Catherine}, citeulike-article-id = {2705375}, citeulike-linkout-0 = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1291220}, citeulike-linkout-1 = {http://dx.doi.org/10.1128/MCB.25.23.10235-10250.2005}, comment = {PMC1291220}, doi = {10.1128/MCB.25.23.10235-10250.2005}, journal = {Molecular and Cellular Biology}, keywords = {erythropoiesis, eto, scl}, posted-at = {2008-04-23 05:47:58}, priority = {0}, title = {ETO-2 Associates with SCL in Erythroid Cells and Megakaryocytes and Provides Repressor Functions in Erythropoiesis}, url = {http://dx.doi.org/10.1128/MCB.25.23.10235-10250.2005}, volume = {25}, year = {2005} }

TY - JOUR ID - schuh_eto-2_2005 L3 - citeulike-article-id:2705375 N2 - Lineage specification and cellular maturation require coordinated regulation of gene expression programs. In large part, this is dependent on the activator and repressor functions of protein complexes associated with tissue-specific transcriptional regulators. In this study, we have used a proteomic approach to characterize multiprotein complexes containing the key hematopoietic regulator SCL in erythroid and megakaryocytic cell lines. One of the novel SCL-interacting proteins identified in both cell types is the transcriptional corepressor ETO-2. Interaction between endogenous proteins was confirmed in primary cells. We then showed that SCL complexes are shared but also significantly differ in the two cell types. Importantly, SCL/ETO-2 interacts with another corepressor, Gfi-1b, in red cells but not megakaryocytes. The SCL/ETO-2/Gfi-1b association is lost during erythroid differentiation of primary fetal liver cells. Genetic studies of erythroid cells show that ETO-2 exerts a repressor effect on SCL target genes. We suggest that, through its association with SCL, ETO-2 represses gene expression in the early stages of erythroid differentiation and that alleviation/modulation of the repressive state is then required for expression of genes necessary for terminal erythroid maturation to proceed. JF - Molecular and Cellular Biology TI - ETO-2 Associates with SCL in Erythroid Cells and Megakaryocytes and Provides Repressor Functions in Erythropoiesis VL - 25 KW - erythropoiesis KW - eto KW - scl N1 - PMC1291220 AU - Schuh, Anna AU - Tipping, Alex AU - Clark, Allison AU - Hamlett, Isla AU - Guyot, Boris AU - Iborra, Francesco AU - Rodriguez, Patrick AU - Strouboulis, John AU - Enver, Tariq AU - Vyas, Paresh AU - Porcher, Catherine PY - 2005/// UR - http://dx.doi.org/10.1128/MCB.25.23.10235-10250.2005 ER -