Caspase 3 activation is essential for efficient influenza virus propagation Export

@article{wurzer_caspase_2003, abstract = {Apoptosis is a hallmark event observed upon infection with many viral pathogens, including influenza A virus. The apoptotic process is executed by a proteolytic system consisting of a family of cysteinyl proteases, termed caspases. Since the consequences of apoptosis induction and caspase activation for the outcome of an influenza virus infection are not clear, we have addressed this issue by interfering with expression or function of a major virus-induced apoptosis effector, caspase 3. Surprisingly, influenza virus propagation was strongly impaired in the presence of an inhibitor that blocks caspase 3 and in cells where caspase 3 was partially knocked down by small interfering RNAs. Consistent with these findings, poor replication efficiencies of influenza A viruses in cells deficient for caspase 3 could be boosted 30-fold by ectopic expression of the protein. Mechanistically, the block in virus propagation appeared to be due to retention of the viral RNP complexes in the nucleus, preventing formation of progeny virus particles. Our findings indicate that caspase 3 activation during the onset of apoptosis is a crucial event for efficient influenza virus propagation.}, author = {Wurzer, Walter J. and Planz, Oliver and Ehrhardt, Christina and Giner, Martin and Silberzahn, Tobias and Pleschka, Stephan and Ludwig, Stephan}, citeulike-article-id = {2705394}, citeulike-linkout-0 = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=158404}, citeulike-linkout-1 = {http://dx.doi.org/10.1093/emboj/cdg279}, comment = {PMC158404}, doi = {10.1093/emboj/cdg279}, journal = {The EMBO Journal}, keywords = {apoptosis, caspase, influenza}, posted-at = {2008-04-23 05:48:02}, priority = {0}, title = {Caspase 3 activation is essential for efficient influenza virus propagation}, url = {http://dx.doi.org/10.1093/emboj/cdg279}, volume = {22}, year = {2003} }

TY - JOUR ID - wurzer_caspase_2003 L3 - citeulike-article-id:2705394 N2 - Apoptosis is a hallmark event observed upon infection with many viral pathogens, including influenza A virus. The apoptotic process is executed by a proteolytic system consisting of a family of cysteinyl proteases, termed caspases. Since the consequences of apoptosis induction and caspase activation for the outcome of an influenza virus infection are not clear, we have addressed this issue by interfering with expression or function of a major virus-induced apoptosis effector, caspase 3. Surprisingly, influenza virus propagation was strongly impaired in the presence of an inhibitor that blocks caspase 3 and in cells where caspase 3 was partially knocked down by small interfering RNAs. Consistent with these findings, poor replication efficiencies of influenza A viruses in cells deficient for caspase 3 could be boosted 30-fold by ectopic expression of the protein. Mechanistically, the block in virus propagation appeared to be due to retention of the viral RNP complexes in the nucleus, preventing formation of progeny virus particles. Our findings indicate that caspase 3 activation during the onset of apoptosis is a crucial event for efficient influenza virus propagation. JF - The EMBO Journal TI - Caspase 3 activation is essential for efficient influenza virus propagation VL - 22 KW - apoptosis KW - caspase KW - influenza N1 - PMC158404 AU - Wurzer, Walter AU - Planz, Oliver AU - Ehrhardt, Christina AU - Giner, Martin AU - Silberzahn, Tobias AU - Pleschka, Stephan AU - Ludwig, Stephan PY - 2003/// UR - http://dx.doi.org/10.1093/emboj/cdg279 ER -