Indoleamine 2,3-Dioxygenase Production by Human Dendritic Cells Results in the Inhibition of T Cell Proliferation Export

@article{hwu_indoleamine_2000, abstract = {Dendritic cells (DCs) play a key role in the activation and regulation of B and T lymphocytes. Production of indoleamine 2,3-dioxygenase (IDO) by macrophages has recently been described to result in inhibition of T cell proliferation through tryptophan degradation. Since DCs can be derived from monocytes, we sought to determine whether DCs could produce IDO which could potentially regulate T cell proliferation. Northern blot analysis of RNA from cultured monocyte-derived human DC revealed that IDO mRNA was induced upon activation with CD40 ligand and IFN-{gamma}. IDO produced from activated DCs was functionally active and capable of metabolizing tryptophan to kynurenine. Activated T cells were also capable of inducing IDO production by DCs, which was inhibited by a neutralizing Ab against IFN-{gamma}. DC production of IDO resulted in inhibition of T cell proliferation, which could be prevented using the IDO inhibitor 1-methyl-DL-tryptophan. These results suggest that activation of DCs induces the production of functional IDO, which causes depletion of tryptophan and subsequent inhibition of T cell proliferation. This may represent a potential mechanism for DCs to regulate the immune response.}, author = {Hwu, Patrick and Du, Mark X. and Lapointe, Rejean and Do, My and Taylor, Milton W. and Young, Howard A.}, citeulike-article-id = {2705398}, citeulike-linkout-0 = {http://www.jimmunol.org/cgi/content/abstract/164/7/3596}, journal = {J Immunol}, keywords = {dc, indo, t\_cell}, month = {April}, pages = {3596--3599}, posted-at = {2008-04-23 05:48:02}, priority = {0}, title = {Indoleamine 2,3-Dioxygenase Production by Human Dendritic Cells Results in the Inhibition of T Cell Proliferation}, url = {http://www.jimmunol.org/cgi/content/abstract/164/7/3596}, volume = {164}, year = {2000} }

TY - JOUR ID - hwu_indoleamine_2000 L3 - citeulike-article-id:2705398 N2 - Dendritic cells (DCs) play a key role in the activation and regulation of B and T lymphocytes. Production of indoleamine 2,3-dioxygenase (IDO) by macrophages has recently been described to result in inhibition of T cell proliferation through tryptophan degradation. Since DCs can be derived from monocytes, we sought to determine whether DCs could produce IDO which could potentially regulate T cell proliferation. Northern blot analysis of RNA from cultured monocyte-derived human DC revealed that IDO mRNA was induced upon activation with CD40 ligand and IFN-{gamma}. IDO produced from activated DCs was functionally active and capable of metabolizing tryptophan to kynurenine. Activated T cells were also capable of inducing IDO production by DCs, which was inhibited by a neutralizing Ab against IFN-{gamma}. DC production of IDO resulted in inhibition of T cell proliferation, which could be prevented using the IDO inhibitor 1-methyl-DL-tryptophan. These results suggest that activation of DCs induces the production of functional IDO, which causes depletion of tryptophan and subsequent inhibition of T cell proliferation. This may represent a potential mechanism for DCs to regulate the immune response. JF - J Immunol EP - 3599 TI - Indoleamine 2,3-Dioxygenase Production by Human Dendritic Cells Results in the Inhibition of T Cell Proliferation VL - 164 SP - 3596 KW - dc KW - indo KW - t_cell AU - Hwu, Patrick AU - Du, Mark AU - Lapointe, Rejean AU - Do, My AU - Taylor, Milton AU - Young, Howard PY - 2000/April// UR - http://www.jimmunol.org/cgi/content/abstract/164/7/3596 ER -