Dectin-1 Is A Major beta-Glucan Receptor On Macrophages Export

@article{brown_dectin-1_2002, abstract = {Zymosan is a {beta}-glucan- and mannan-rich particle that is widely used as a cellular activator for examining the numerous responses effected by phagocytes. The macrophage mannose receptor (MR) and complement receptor 3 (CR3) have historically been considered the major macrophage lectins involved in the nonopsonic recognition of these yeast-derived particles. Using specific carbohydrate inhibitors, we show that a {beta}-glucan receptor, but not the MR, is a predominant receptor involved in this process. Furthermore, nonopsonic zymosan binding was unaffected by genetic CD11b deficiency or a blocking monoclonal antibody (mAb) against CR3, demonstrating that CR3 was not the {beta}-glucan receptor mediating this activity. To address the role of the recently described {beta}-glucan receptor, Dectin-1, we generated a novel anti-Dectin-1 mAb, 2A11. Using this mAb, we show here that Dectin-1 was almost exclusively responsible for the {beta}-glucan-dependent, nonopsonic recognition of zymosan by primary macro-phages. These findings define Dectin-1 as the leukocyte {beta}-glucan receptor, first described over 50 years ago, and resolves the long-standing controversy regarding the identity of this important molecule. Furthermore, these results identify Dectin-1 as a new target for examining the immunomodulatory properties of {beta}-glucans for therapeutic drug design.}, author = {Brown, Gordon D. and Taylor, Philip R. and Reid, Delyth M. and Willment, Janet A. and Williams, David L. and Pomares, Luisa M. and Wong, Simon Y. C. and Gordon, Siamon}, citeulike-article-id = {2705413}, citeulike-linkout-0 = {http://www.jem.org/cgi/content/abstract/196/3/407}, citeulike-linkout-1 = {http://dx.doi.org/10.1084/jem.20020470}, doi = {10.1084/jem.20020470}, journal = {J. Exp. Med.}, keywords = {beta-glucan, dectin-1, macrophage}, month = {August}, pages = {407--412}, posted-at = {2008-04-23 05:48:09}, priority = {0}, title = {Dectin-1 Is A Major {beta}-Glucan Receptor On Macrophages}, url = {http://dx.doi.org/10.1084/jem.20020470}, volume = {196}, year = {2002} }

TY - JOUR ID - brown_dectin-1_2002 L3 - citeulike-article-id:2705413 N2 - Zymosan is a {beta}-glucan- and mannan-rich particle that is widely used as a cellular activator for examining the numerous responses effected by phagocytes. The macrophage mannose receptor (MR) and complement receptor 3 (CR3) have historically been considered the major macrophage lectins involved in the nonopsonic recognition of these yeast-derived particles. Using specific carbohydrate inhibitors, we show that a {beta}-glucan receptor, but not the MR, is a predominant receptor involved in this process. Furthermore, nonopsonic zymosan binding was unaffected by genetic CD11b deficiency or a blocking monoclonal antibody (mAb) against CR3, demonstrating that CR3 was not the {beta}-glucan receptor mediating this activity. To address the role of the recently described {beta}-glucan receptor, Dectin-1, we generated a novel anti-Dectin-1 mAb, 2A11. Using this mAb, we show here that Dectin-1 was almost exclusively responsible for the {beta}-glucan-dependent, nonopsonic recognition of zymosan by primary macro-phages. These findings define Dectin-1 as the leukocyte {beta}-glucan receptor, first described over 50 years ago, and resolves the long-standing controversy regarding the identity of this important molecule. Furthermore, these results identify Dectin-1 as a new target for examining the immunomodulatory properties of {beta}-glucans for therapeutic drug design. JF - J. Exp. Med. EP - 412 TI - Dectin-1 Is A Major {beta}-Glucan Receptor On Macrophages VL - 196 SP - 407 KW - beta-glucan KW - dectin-1 KW - macrophage AU - Brown, Gordon AU - Taylor, Philip AU - Reid, Delyth AU - Willment, Janet AU - Williams, David AU - Pomares, Luisa AU - Wong, Simon AU - Gordon, Siamon PY - 2002/August// UR - http://dx.doi.org/10.1084/jem.20020470 ER -