Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions Export

@article{reid_expression_2004, abstract = {Dectin-1 is a pathogen-recognition receptor on macrophages (M{Phi}s), neutrophils, and dendritic cells (DCs). On M{Phi}s and bone marrow-derived DCs, it has been shown to mediate the nonopsonic recognition of and response to soluble and particulate yeast {beta}-glucans. We have optimized the immunohistochemical detection of Dectin-1 and demonstrated its expression on neutrophils, subpopulations of M{Phi}s in splenic red and white pulp, alveolar M{Phi}s, Kupffer cells, and M{Phi}s and DCs in the lamina propria of gut villi. This is consistent with its role in pathogen surveillance. A significant proportion of CD11c+ splenic DCs expressed Dectin-1, but expression was not restricted to any one subset. Dectin-1 expression was low on resident M{Phi}s and DCs of skin and was not detected on resident M{Phi}s or DCs in kidney, heart, brain, or eye. The proposed, additional role of Dectin-1 as a coreceptor for T cell activation is supported by its expression on DCs in the T cell areas of the spleen and lymph nodes. Strong expression of Dectin-1 on subpopulations of M{Phi}s and DCs in the medullary and corticomedullary regions of the thymus suggests a role distinct from pathogen recognition. Tissue localization thus revealed potential roles of Dectin-1 in leukocyte interactions during innate immune responses and T cell development.}, author = {Reid, Delyth M. and Montoya, Maria and Taylor, Philip R. and Borrow, Persephone and Gordon, Siamon and Brown, Gordon D. and Wong, Simon Y. C.}, citeulike-article-id = {2705432}, citeulike-linkout-0 = {http://www.jleukbio.org/cgi/content/abstract/76/1/86}, citeulike-linkout-1 = {http://dx.doi.org/10.1189/jlb.0104031}, doi = {10.1189/jlb.0104031}, journal = {J Leukoc Biol}, keywords = {beta-glucan, dectin-1}, month = {July}, pages = {86--94}, posted-at = {2008-04-23 05:48:11}, priority = {0}, title = {Expression of the {beta}-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions}, url = {http://dx.doi.org/10.1189/jlb.0104031}, volume = {76}, year = {2004} }

TY - JOUR ID - reid_expression_2004 L3 - citeulike-article-id:2705432 N2 - Dectin-1 is a pathogen-recognition receptor on macrophages (M{Phi}s), neutrophils, and dendritic cells (DCs). On M{Phi}s and bone marrow-derived DCs, it has been shown to mediate the nonopsonic recognition of and response to soluble and particulate yeast {beta}-glucans. We have optimized the immunohistochemical detection of Dectin-1 and demonstrated its expression on neutrophils, subpopulations of M{Phi}s in splenic red and white pulp, alveolar M{Phi}s, Kupffer cells, and M{Phi}s and DCs in the lamina propria of gut villi. This is consistent with its role in pathogen surveillance. A significant proportion of CD11c+ splenic DCs expressed Dectin-1, but expression was not restricted to any one subset. Dectin-1 expression was low on resident M{Phi}s and DCs of skin and was not detected on resident M{Phi}s or DCs in kidney, heart, brain, or eye. The proposed, additional role of Dectin-1 as a coreceptor for T cell activation is supported by its expression on DCs in the T cell areas of the spleen and lymph nodes. Strong expression of Dectin-1 on subpopulations of M{Phi}s and DCs in the medullary and corticomedullary regions of the thymus suggests a role distinct from pathogen recognition. Tissue localization thus revealed potential roles of Dectin-1 in leukocyte interactions during innate immune responses and T cell development. JF - J Leukoc Biol EP - 94 TI - Expression of the {beta}-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions VL - 76 SP - 86 KW - beta-glucan KW - dectin-1 AU - Reid, Delyth AU - Montoya, Maria AU - Taylor, Philip AU - Borrow, Persephone AU - Gordon, Siamon AU - Brown, Gordon AU - Wong, Simon PY - 2004/July// UR - http://dx.doi.org/10.1189/jlb.0104031 ER -