An Anti-Idiotype Vaccine Elicits a Specific Response to N-Glycolyl Sialic Acid Residues of Glycoconjugates in Melanoma Patients Export

@article{citeulike:2752906, abstract = {We generated the 1E10 {gamma}-type anti-idiotype mAb (Ab2) specific to an Ab1 mAb able to react specifically with N-glycolyl-containing gangliosides and with Ags expressed on human melanoma and breast carcinoma cells. This Ab2 mAb induced an Ab response in animal models sharing immunochemically defined idiotopes with the Ab1. The treatment of tumor-bearing mice with 1E10 mAb induced a strong antitumor activity. A clinical trial was conducted in 20 patients with advanced malignant melanoma. Patients were treated with six intradermal injections of aluminum hydroxide-precipitated 1E10 anti-Id mAb given at 2-wk intervals. Sixteen of the 17 patients who received at least four doses of the anti-Id vaccine develop Ab3 Abs capable of inhibiting Ab2 binding to Ab1 (Ab3Id+). In contrast to the incapacity of 1E10 mAb to generate Ab3 Abs with the same antigenic specificity as the Ab1 mAb in mice, a very specific and strong Ab3 response against N-glycolyl-containing gangliosides was induced in 16 patients (Ab3Ag+). No evidence of serious or unexpected adverse effects has been observed in this clinical trial. 1E10 anti-Id vaccine was safe, well tolerated, and immunologically effective, with most patients being able to generate a specific immune response against 1E10 and Neu-glycolyl-GM3 ganglioside.}, author = {Alfonso, Mauro and Diaz, Alain and Hernandez, Ana M. and Perez, Alexis and Rodriguez, Edmundo and Bitton, Roberto and Perez, Rolando and Vazquez, Ana M.}, citeulike-article-id = {2752906}, citeulike-linkout-0 = {http://www.jimmunol.org/cgi/content/abstract/168/5/2523}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11859147}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11859147}, day = {1}, journal = {J Immunol}, keywords = {antibody, cancer, vaccine}, month = {March}, number = {5}, pages = {2523--2529}, posted-at = {2008-05-04 06:59:38}, priority = {3}, title = {An Anti-Idiotype Vaccine Elicits a Specific Response to N-Glycolyl Sialic Acid Residues of Glycoconjugates in Melanoma Patients}, url = {http://www.jimmunol.org/cgi/content/abstract/168/5/2523}, volume = {168}, year = {2002} }

TY - JOUR ID - citeulike:2752906 L3 - citeulike-article-id:2752906 N2 - We generated the 1E10 {gamma}-type anti-idiotype mAb (Ab2) specific to an Ab1 mAb able to react specifically with N-glycolyl-containing gangliosides and with Ags expressed on human melanoma and breast carcinoma cells. This Ab2 mAb induced an Ab response in animal models sharing immunochemically defined idiotopes with the Ab1. The treatment of tumor-bearing mice with 1E10 mAb induced a strong antitumor activity. A clinical trial was conducted in 20 patients with advanced malignant melanoma. Patients were treated with six intradermal injections of aluminum hydroxide-precipitated 1E10 anti-Id mAb given at 2-wk intervals. Sixteen of the 17 patients who received at least four doses of the anti-Id vaccine develop Ab3 Abs capable of inhibiting Ab2 binding to Ab1 (Ab3Id+). In contrast to the incapacity of 1E10 mAb to generate Ab3 Abs with the same antigenic specificity as the Ab1 mAb in mice, a very specific and strong Ab3 response against N-glycolyl-containing gangliosides was induced in 16 patients (Ab3Ag+). No evidence of serious or unexpected adverse effects has been observed in this clinical trial. 1E10 anti-Id vaccine was safe, well tolerated, and immunologically effective, with most patients being able to generate a specific immune response against 1E10 and Neu-glycolyl-GM3 ganglioside. IS - 5 JF - J Immunol EP - 2529 TI - An Anti-Idiotype Vaccine Elicits a Specific Response to N-Glycolyl Sialic Acid Residues of Glycoconjugates in Melanoma Patients VL - 168 SP - 2523 KW - antibody KW - cancer KW - vaccine AU - Alfonso, Mauro AU - Diaz, Alain AU - Hernandez, Ana AU - Perez, Alexis AU - Rodriguez, Edmundo AU - Bitton, Roberto AU - Perez, Rolando AU - Vazquez, Ana PY - 2002/03/01/ UR - http://www.jimmunol.org/cgi/content/abstract/168/5/2523 ER -