Cutting Edge: NKT Cells Constitutively Express IL-23 Receptor and RORgammat and Rapidly Produce IL-17 upon Receptor Ligation in an IL-6-Independent Fashion Export

@article{citeulike:2762866, abstract = {Th17 cells require IL-6 and TGF for lineage commitment and IL-23 for maintenance. Unexpectedly, naive IL-6-/- splenocytes stimulated with anti-CD3 and IL-23 produced normal amounts of IL-17 during the first 24 h of culture. These rapid IL-6-independent IL-17 producers were identified as predominantly DX5+ TCR+ NKT cells, and a comparable response could be found using the invariant NKT-specific ligand {alpha}-galactosylceramide. Human NKT cells also produced IL-17. NKT cells constitutively expressed IL-23R and ROR{gamma}t. Ligation of either TCR or IL-23R triggered IL-17 production and both together had a synergistic effect, suggesting independent but convergent pathways. IL-17 production was not restricted to a particular subset of NKT cells but they were NK1.1 negative. Importantly, in vivo administration of {alpha}-galactosylceramide triggered a rapid IL-17 response in the spleen. These data suggest an important biological role for innate IL-17 production by NKT cells that is rapid and precedes the adaptive IL-17 response.}, author = {Rachitskaya, Aleksandra V. and Hansen, Anna M. and Horai, Reiko and Li, Zhuqing and Villasmil, Rafael and Luger, Dror and Nussenblatt, Robert B. and Caspi, Rachel R.}, citeulike-article-id = {2762866}, citeulike-linkout-0 = {http://www.jimmunol.org/cgi/content/abstract/180/8/5167}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18390697}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18390697}, day = {15}, journal = {J Immunol}, keywords = {il-17, il-23, il-6, interleukin, nkt, ror}, month = {April}, number = {8}, pages = {5167--5171}, posted-at = {2008-05-06 21:50:10}, priority = {3}, title = {Cutting Edge: NKT Cells Constitutively Express IL-23 Receptor and ROR{gamma}t and Rapidly Produce IL-17 upon Receptor Ligation in an IL-6-Independent Fashion}, url = {http://www.jimmunol.org/cgi/content/abstract/180/8/5167}, volume = {180}, year = {2008} }

TY - JOUR ID - citeulike:2762866 L3 - citeulike-article-id:2762866 N2 - Th17 cells require IL-6 and TGF for lineage commitment and IL-23 for maintenance. Unexpectedly, naive IL-6-/- splenocytes stimulated with anti-CD3 and IL-23 produced normal amounts of IL-17 during the first 24 h of culture. These rapid IL-6-independent IL-17 producers were identified as predominantly DX5+ TCR+ NKT cells, and a comparable response could be found using the invariant NKT-specific ligand {alpha}-galactosylceramide. Human NKT cells also produced IL-17. NKT cells constitutively expressed IL-23R and ROR{gamma}t. Ligation of either TCR or IL-23R triggered IL-17 production and both together had a synergistic effect, suggesting independent but convergent pathways. IL-17 production was not restricted to a particular subset of NKT cells but they were NK1.1 negative. Importantly, in vivo administration of {alpha}-galactosylceramide triggered a rapid IL-17 response in the spleen. These data suggest an important biological role for innate IL-17 production by NKT cells that is rapid and precedes the adaptive IL-17 response. IS - 8 JF - J Immunol EP - 5171 TI - Cutting Edge: NKT Cells Constitutively Express IL-23 Receptor and ROR{gamma}t and Rapidly Produce IL-17 upon Receptor Ligation in an IL-6-Independent Fashion VL - 180 SP - 5167 KW - il-17 KW - il-23 KW - il-6 KW - interleukin KW - nkt KW - ror AU - Rachitskaya, Aleksandra AU - Hansen, Anna AU - Horai, Reiko AU - Li, Zhuqing AU - Villasmil, Rafael AU - Luger, Dror AU - Nussenblatt, Robert AU - Caspi, Rachel PY - 2008/04/15/ UR - http://www.jimmunol.org/cgi/content/abstract/180/8/5167 ER -