Null mutations in human and mouse orthologs frequently result in different phenotypes Export

@article{citeulike:2772738, abstract = {10.1073/pnas.0800387105 One-to-one orthologous genes of relatively closely related species are widely assumed to have similar functions and cause similar phenotypes when deleted from the genome. Although this assumption is the foundation of comparative genomics and the basis for the use of model organisms to study human biology and disease, its validity is known only from anecdotes rather than from systematic examination. Comparing documented phenotypes of null mutations in humans and mice, we find that >20\% of human essential genes have nonessential mouse orthologs. These changes of gene essentiality appear to be associated with adaptive evolution at the protein-sequence, but not gene-expression, level. Proteins localized to the vacuole, a cellular compartment for waste management, are highly enriched among essentiality-changing genes. It is probable that the evolution of the prolonged life history in humans required enhanced waste management for proper cellular function until the time of reproduction, which rendered these vacuole proteins essential and generated selective pressures for their improvement. If our gene sample represents the entire genome, our results would mean frequent changes of phenotypic effects of one-to-one orthologous genes even between relatively closely related species, a possibility that should be considered in comparative genomic studies and in making cross-species inferences of gene function and phenotypic effect.}, address = {Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109.}, author = {Liao, Ben-Yang and Zhang, Jianzhi}, citeulike-article-id = {2772738}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0800387105}, citeulike-linkout-1 = {http://www.pnas.org/content/105/19/6987.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/19/6987.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/18458337}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=18458337}, day = {13}, doi = {10.1073/pnas.0800387105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences}, keywords = {human, mammals, mouse, nullmutation, phenotypes}, month = {May}, number = {19}, pages = {6987--6992}, posted-at = {2009-11-02 08:56:03}, priority = {5}, title = {Null mutations in human and mouse orthologs frequently result in different phenotypes}, url = {http://dx.doi.org/10.1073/pnas.0800387105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:2772738 L3 - citeulike-article-id:2772738 N2 - 10.1073/pnas.0800387105 One-to-one orthologous genes of relatively closely related species are widely assumed to have similar functions and cause similar phenotypes when deleted from the genome. Although this assumption is the foundation of comparative genomics and the basis for the use of model organisms to study human biology and disease, its validity is known only from anecdotes rather than from systematic examination. Comparing documented phenotypes of null mutations in humans and mice, we find that >20% of human essential genes have nonessential mouse orthologs. These changes of gene essentiality appear to be associated with adaptive evolution at the protein-sequence, but not gene-expression, level. Proteins localized to the vacuole, a cellular compartment for waste management, are highly enriched among essentiality-changing genes. It is probable that the evolution of the prolonged life history in humans required enhanced waste management for proper cellular function until the time of reproduction, which rendered these vacuole proteins essential and generated selective pressures for their improvement. If our gene sample represents the entire genome, our results would mean frequent changes of phenotypic effects of one-to-one orthologous genes even between relatively closely related species, a possibility that should be considered in comparative genomic studies and in making cross-species inferences of gene function and phenotypic effect. IS - 19 JF - Proceedings of the National Academy of Sciences SN - 1091-6490 AD - Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109. EP - 6992 TI - Null mutations in human and mouse orthologs frequently result in different phenotypes VL - 105 SP - 6987 KW - human KW - mammals KW - mouse KW - nullmutation KW - phenotypes AU - Liao, Ben-Yang AU - Zhang, Jianzhi PY - 2008/05/13/ UR - http://dx.doi.org/10.1073/pnas.0800387105 ER -