A Ligation-Independent Cloning Tobacco Rattle Virus Vector for High-Throughput Virus-Induced Gene Silencing Identifies Roles for NbMADS4-1 and -2 in Floral Development Export

@article{citeulike:3057981, abstract = {Virus-induced gene silencing (VIGS) is a widely used, powerful technique for reverse genetics. VIGS vectors derived from the Tobacco rattle virus (TRV) are among the most popular for VIGS. We have developed a TRV RNA2 vector that allows the insertion of gene silencing fragments by ligation-independent cloning (LIC). This new vector has several advantages over previous vectors, particularly for applications involving the analysis of large numbers of sequences, since TRV-LIC vectors containing the desired insert are obtained with 100\% efficiency. Importantly, this vector allows the high-throughput cloning of silencing fragments without the use of costly enzymes required for recombination, as is the case with GATEWAY-based vectors. We generated a collection of silencing vectors based on 400 tomato (Solanum lycopersicum) expressed sequence tags in this TRV-LIC background. We have used this vector to identify roles for SlMADS1 and its Nicotiana benthamiana homologs, NbMADS4-1 and -2 in flowering. We find that NbMADS4-1 and -2 act nonredundantly in floral development and silencing of either gene results in loss of organ identity. This TRV-LIC vector should be a valuable resource to the plant community. 10.1104/pp.107.107391}, author = {Dong, Yiyu and Burch-Smith, Tessa M. and Liu, Yule and Mamillapalli, Padmavathi and Dinesh-Kumar, Savithramma P.}, citeulike-article-id = {3057981}, citeulike-linkout-0 = {http://dx.doi.org/10.1104/pp.107.107391}, citeulike-linkout-1 = {http://www.plantphysiol.org/cgi/content/abstract/145/4/1161}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17932306}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17932306}, day = {1}, doi = {10.1104/pp.107.107391}, journal = {Plant Physiol.}, keywords = {lic}, month = {December}, number = {4}, pages = {1161--1170}, posted-at = {2008-07-30 00:26:59}, priority = {2}, title = {A Ligation-Independent Cloning Tobacco Rattle Virus Vector for High-Throughput Virus-Induced Gene Silencing Identifies Roles for NbMADS4-1 and -2 in Floral Development}, url = {http://dx.doi.org/10.1104/pp.107.107391}, volume = {145}, year = {2007} }

TY - JOUR ID - citeulike:3057981 L3 - citeulike-article-id:3057981 N2 - Virus-induced gene silencing (VIGS) is a widely used, powerful technique for reverse genetics. VIGS vectors derived from the Tobacco rattle virus (TRV) are among the most popular for VIGS. We have developed a TRV RNA2 vector that allows the insertion of gene silencing fragments by ligation-independent cloning (LIC). This new vector has several advantages over previous vectors, particularly for applications involving the analysis of large numbers of sequences, since TRV-LIC vectors containing the desired insert are obtained with 100% efficiency. Importantly, this vector allows the high-throughput cloning of silencing fragments without the use of costly enzymes required for recombination, as is the case with GATEWAY-based vectors. We generated a collection of silencing vectors based on 400 tomato (Solanum lycopersicum) expressed sequence tags in this TRV-LIC background. We have used this vector to identify roles for SlMADS1 and its Nicotiana benthamiana homologs, NbMADS4-1 and -2 in flowering. We find that NbMADS4-1 and -2 act nonredundantly in floral development and silencing of either gene results in loss of organ identity. This TRV-LIC vector should be a valuable resource to the plant community. 10.1104/pp.107.107391 IS - 4 JF - Plant Physiol. EP - 1170 TI - A Ligation-Independent Cloning Tobacco Rattle Virus Vector for High-Throughput Virus-Induced Gene Silencing Identifies Roles for NbMADS4-1 and -2 in Floral Development VL - 145 SP - 1161 KW - lic AU - Dong, Yiyu AU - Burch-Smith, Tessa AU - Liu, Yule AU - Mamillapalli, Padmavathi AU - Dinesh-Kumar, Savithramma PY - 2007/12/01/ UR - http://dx.doi.org/10.1104/pp.107.107391 ER -