Proteomic Profiling of Plasmodium Sporozoite Maturation Identifies New Proteins Essential for Parasite Development and Infectivity Export

@article{citeulike:3469257, abstract = {Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite stages from the mosquito—early and late oocysts containing midgut sporozoites, and the mature, infectious salivary gland sporozoites. Despite the morphological similarity between midgut and salivary gland sporozoites, their proteomes are markedly different, in agreement with their increase in hepatocyte infectivity. The different sporozoite proteomes contain a large number of stage specific proteins whose annotation suggest an involvement in sporozoite maturation, motility, infection of the human host and associated metabolic adjustments. Analyses of proteins identified in the P. falciparum sporozoite proteomes by orthologous gene disruption in the rodent malaria parasite, P. berghei, revealed three previously uncharacterized Plasmodium proteins that appear to be essential for sporozoite development at distinct points of maturation in the mosquito. This study sheds light on the development and maturation of the malaria parasite in an Anopheles mosquito and also identifies proteins that may be essential for sporozoite infectivity to humans.}, author = {Lasonder, Edwin and Janse, Chris J. and van Gemert, Geert-Jan and Mair, Gunnar R. and Vermunt, Adriaan M. and Douradinha, Bruno G. and van Noort, Vera and Huynen, Martijn A. and Luty, Adrian J. and Kroeze, Hans and Khan, Shahid M. and Sauerwein, Robert W. and Waters, Andrew P. and Mann, Matthias and Stunnenberg, Hendrik G.}, citeulike-article-id = {3469257}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.ppat.1000195}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18974882}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18974882}, day = {31}, doi = {10.1371/journal.ppat.1000195}, journal = {PLoS Pathog}, keywords = {malaria, proteomics}, month = {October}, number = {10}, pages = {e1000195+}, posted-at = {2008-10-31 13:42:15}, priority = {2}, publisher = {Public Library of Science}, title = {Proteomic Profiling of Plasmodium Sporozoite Maturation Identifies New Proteins Essential for Parasite Development and Infectivity}, url = {http://dx.doi.org/10.1371/journal.ppat.1000195}, volume = {4}, year = {2008} }

TY - JOUR ID - citeulike:3469257 L3 - citeulike-article-id:3469257 N2 - Plasmodium falciparum sporozoites that develop and mature inside an Anopheles mosquito initiate a malaria infection in humans. Here we report the first proteomic comparison of different parasite stages from the mosquito—early and late oocysts containing midgut sporozoites, and the mature, infectious salivary gland sporozoites. Despite the morphological similarity between midgut and salivary gland sporozoites, their proteomes are markedly different, in agreement with their increase in hepatocyte infectivity. The different sporozoite proteomes contain a large number of stage specific proteins whose annotation suggest an involvement in sporozoite maturation, motility, infection of the human host and associated metabolic adjustments. Analyses of proteins identified in the P. falciparum sporozoite proteomes by orthologous gene disruption in the rodent malaria parasite, P. berghei, revealed three previously uncharacterized Plasmodium proteins that appear to be essential for sporozoite development at distinct points of maturation in the mosquito. This study sheds light on the development and maturation of the malaria parasite in an Anopheles mosquito and also identifies proteins that may be essential for sporozoite infectivity to humans. IS - 10 JF - PLoS Pathog TI - Proteomic Profiling of Plasmodium Sporozoite Maturation Identifies New Proteins Essential for Parasite Development and Infectivity PB - Public Library of Science VL - 4 SP - e1000195 KW - malaria KW - proteomics AU - Lasonder, Edwin AU - Janse, Chris AU - van Gemert, Geert-Jan AU - Mair, Gunnar AU - Vermunt, Adriaan AU - Douradinha, Bruno AU - van Noort, Vera AU - Huynen, Martijn AU - Luty, Adrian AU - Kroeze, Hans AU - Khan, Shahid AU - Sauerwein, Robert AU - Waters, Andrew AU - Mann, Matthias AU - Stunnenberg, Hendrik PY - 2008/10/31/ UR - http://dx.doi.org/10.1371/journal.ppat.1000195 ER -