A novel non-protein-coding infection-specific gene family is clustered throughout the genome of Phytophthora infestans Export

@article{citeulike:6076310, abstract = {Phytophthora infestans is the cause of late blight, a devastating and re-emerging disease of potato. Significant advances have been made in understanding the biology of P. infestans, and in the development of molecular tools to study this oomycete. Nevertheless, little is known about the molecular bases of the establishment or development of disease in this hemibiotrophic pathogen. Suppression subtractive hybridization (SSH) was used to generate cDNA enriched for sequences upregulated during potato infection. To identify pathogen-derived cDNAs, and eliminate host sequences from further study, SSH cDNA was hybridized to a P. infestans bacterial artificial chromosome library. A new gene family was identified called Pinci1, comprising more than 400 members arranged in clusters of up to nine copies throughout the P. infestans draft genome sequence. Real-time RT-PCR was used to quantify the expression of five classes of transcript within the family, relative to the constitutively expressed PiactA gene, and it revealed them to be significantly upregulated from 12 to 33 h post-inoculation, a period defining the biotrophic phase of infection. Computational analysis of sequences suggested that transcripts were non-protein coding, and this was confirmed by transient expression of FLAG-tagged ORFs in P. infestans. 10.1099/mic.0.2006/002220-0}, author = {Avrova, Anna O. and Whisson, Stephen C. and Pritchard, Leighton and Venter, Eduard and De Luca, Sergio and Hein, Ingo and Birch, Paul R. J.}, citeulike-article-id = {6076310}, citeulike-linkout-0 = {http://dx.doi.org/10.1099/mic.0.2006/002220-0}, citeulike-linkout-1 = {http://mic.sgmjournals.org/content/153/3/747.abstract}, citeulike-linkout-2 = {http://mic.sgmjournals.org/content/153/3/747.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/17322195}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=17322195}, day = {1}, doi = {10.1099/mic.0.2006/002220-0}, journal = {Microbiology}, keywords = {ncrna}, month = {March}, number = {3}, pages = {747--759}, posted-at = {2009-11-05 19:40:29}, priority = {2}, title = {A novel non-protein-coding infection-specific gene family is clustered throughout the genome of Phytophthora infestans}, url = {http://dx.doi.org/10.1099/mic.0.2006/002220-0}, volume = {153}, year = {2007} }

TY - JOUR ID - citeulike:6076310 L3 - citeulike-article-id:6076310 N2 - Phytophthora infestans is the cause of late blight, a devastating and re-emerging disease of potato. Significant advances have been made in understanding the biology of P. infestans, and in the development of molecular tools to study this oomycete. Nevertheless, little is known about the molecular bases of the establishment or development of disease in this hemibiotrophic pathogen. Suppression subtractive hybridization (SSH) was used to generate cDNA enriched for sequences upregulated during potato infection. To identify pathogen-derived cDNAs, and eliminate host sequences from further study, SSH cDNA was hybridized to a P. infestans bacterial artificial chromosome library. A new gene family was identified called Pinci1, comprising more than 400 members arranged in clusters of up to nine copies throughout the P. infestans draft genome sequence. Real-time RT-PCR was used to quantify the expression of five classes of transcript within the family, relative to the constitutively expressed PiactA gene, and it revealed them to be significantly upregulated from 12 to 33 h post-inoculation, a period defining the biotrophic phase of infection. Computational analysis of sequences suggested that transcripts were non-protein coding, and this was confirmed by transient expression of FLAG-tagged ORFs in P. infestans. 10.1099/mic.0.2006/002220-0 IS - 3 JF - Microbiology EP - 759 TI - A novel non-protein-coding infection-specific gene family is clustered throughout the genome of Phytophthora infestans VL - 153 SP - 747 KW - ncrna AU - Avrova, Anna AU - Whisson, Stephen AU - Pritchard, Leighton AU - Venter, Eduard AU - De Luca, Sergio AU - Hein, Ingo AU - Birch, Paul PY - 2007/03/01/ UR - http://dx.doi.org/10.1099/mic.0.2006/002220-0 ER -