G Protein-Coupled Receptor 30 and Estrogen Receptor ∝ Are Involved in the Proliferative Effects Induced by Atrazine in Ovarian Cancer Cells Export

@article{citeulike:3036974, abstract = {Abstract Background: Atrazine, one of the most common pesticide contaminants, has been shown to upregulate aromatase activity in certain estrogen-sensitive tumors without binding or activating the estrogen receptor (ER). Recent investigations have demonstrated that the orphan G-protein coupled receptor 30 (GPR30), which is structurally unrelated to the ER, mediates rapid actions of 17β- estradiol (E2) and environmental estrogens. Objectives: Given the ability of atrazine to exert estrogen-like activity in cancer cells, we evaluated the potential of atrazine to signal through GPR30 in stimulating biological responses in cancer cells. Methods and Results: Atrazine did not transactivate the endogenous ERα in different cancer cell contexts or chimeric proteins encoding the ERα and ERβ hormone binding domain in gene reporter assays. Moreover, atrazine neither regulated the expression of ERα, nor stimulated aromatase activity. Interestingly, atrazine induced ERK phosphorylation and the expression of estrogen target genes. Using specific signaling inhibitors and gene silencing, we demonstrated that atrazine stimulated the proliferation of ovarian cancer cells through the GPR30-EGFR transduction pathway and the involvement of ERα. Conclusions: Our results indicate a novel mechanism through which atrazine may exert relevant biological effects in cancer cells. On the basis of the present data atrazine should be included among the environmental contaminants potentially able to signal via GPR30 in eliciting estrogenic action.}, author = {Albanito, Lidia and Lappano, Rosamaria and Madeo, Antonio and Chimento, Adele and Prossnitz, Eric R. and Cappello, Anna R. and Dolce, Vincenza and Abonante, Sergio and Pezzi, Vincenzo and Maggiolini, Marcello}, citeulike-article-id = {3036974}, citeulike-linkout-0 = {http://www.ehponline.org/docs/2008/11297/abstract.html}, day = {22}, keywords = {atrazine, protein-coupled, receptor}, month = {July}, posted-at = {2008-07-23 15:28:24}, priority = {3}, title = {G Protein-Coupled Receptor 30 and Estrogen Receptor ∝ Are Involved in the Proliferative Effects Induced by Atrazine in Ovarian Cancer Cells}, url = {http://www.ehponline.org/docs/2008/11297/abstract.html}, year = {2008} }

TY - JOUR ID - citeulike:3036974 L3 - citeulike-article-id:3036974 N2 - Abstract Background: Atrazine, one of the most common pesticide contaminants, has been shown to upregulate aromatase activity in certain estrogen-sensitive tumors without binding or activating the estrogen receptor (ER). Recent investigations have demonstrated that the orphan G-protein coupled receptor 30 (GPR30), which is structurally unrelated to the ER, mediates rapid actions of 17β- estradiol (E2) and environmental estrogens. Objectives: Given the ability of atrazine to exert estrogen-like activity in cancer cells, we evaluated the potential of atrazine to signal through GPR30 in stimulating biological responses in cancer cells. Methods and Results: Atrazine did not transactivate the endogenous ERα in different cancer cell contexts or chimeric proteins encoding the ERα and ERβ hormone binding domain in gene reporter assays. Moreover, atrazine neither regulated the expression of ERα, nor stimulated aromatase activity. Interestingly, atrazine induced ERK phosphorylation and the expression of estrogen target genes. Using specific signaling inhibitors and gene silencing, we demonstrated that atrazine stimulated the proliferation of ovarian cancer cells through the GPR30-EGFR transduction pathway and the involvement of ERα. Conclusions: Our results indicate a novel mechanism through which atrazine may exert relevant biological effects in cancer cells. On the basis of the present data atrazine should be included among the environmental contaminants potentially able to signal via GPR30 in eliciting estrogenic action. TI - G Protein-Coupled Receptor 30 and Estrogen Receptor ∝ Are Involved in the Proliferative Effects Induced by Atrazine in Ovarian Cancer Cells KW - atrazine KW - protein-coupled KW - receptor AU - Albanito, Lidia AU - Lappano, Rosamaria AU - Madeo, Antonio AU - Chimento, Adele AU - Prossnitz, Eric AU - Cappello, Anna AU - Dolce, Vincenza AU - Abonante, Sergio AU - Pezzi, Vincenzo AU - Maggiolini, Marcello PY - 2008/07/22/ UR - http://www.ehponline.org/docs/2008/11297/abstract.html ER -