ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation. Export

@article{citeulike:3439202, abstract = {Peripheral initiators of muscle pain are virtually unknown, but likely key to development of chronic pain after muscle insult. The current study tested the hypothesis that ASIC3 in muscle is necessary for development of cutaneous mechanical, but not heat, hyperalgesia induced by muscle inflammation. Using mechanical and heat stimuli, we assessed behavioral responses in ASIC3-/- and ASIC3+/+ mice after induction of carrageenan muscle inflammation. ASIC3-/- mice did not develop cutaneous mechanical hyperalgesia after muscle inflammation when compared to ASIC3+/+ mice; heat hyperalgesia developed similarly between groups. We then tested if the phenotype could be rescued in ASIC3-/- mice by using a recombinant herpes virus vector to express ASIC3 in skin (where testing occurred) or muscle (where inflammation occurred). Infection of mouse DRG neurons with ASIC3-encoding virus resulted in functional expression of ASICs. Injection of ASIC3-encoding virus into muscle or skin of ASIC3-/- mice resulted in ASIC3 mRNA in DRG and protein expression in DRG and the peripheral injection site. Injection of ASIC3-encoding virus into muscle, but not skin, resulted in development of mechanical hyperalgesia similar to that observed in ASIC3+/+ mice. Thus, ASIC3 in primary afferent fibers innervating muscle is critical to development of hyperalgesia that results from muscle insult.}, author = {Sluka, K. A. and Radhakrishnan, R. and Benson, C. J. and Eshcol, J. O. and Price, M. P. and Babinski, K. and Audette, K. M. and Yeomans, D. C. and Wilson, S. P.}, citeulike-article-id = {3439202}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.pain.2006.09.038}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17134831}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17134831}, doi = {10.1016/j.pain.2006.09.038}, issn = {1872-6623}, journal = {Pain}, keywords = {asic3, heat\_hyperalgesia, mechanical\_hyperalgesia, muscle\_inflammation}, month = {May}, number = {1-2}, pages = {102--112}, posted-at = {2008-10-22 12:13:26}, priority = {3}, title = {ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation.}, url = {http://dx.doi.org/10.1016/j.pain.2006.09.038}, volume = {129}, year = {2007} }

TY - JOUR ID - citeulike:3439202 L3 - citeulike-article-id:3439202 N2 - Peripheral initiators of muscle pain are virtually unknown, but likely key to development of chronic pain after muscle insult. The current study tested the hypothesis that ASIC3 in muscle is necessary for development of cutaneous mechanical, but not heat, hyperalgesia induced by muscle inflammation. Using mechanical and heat stimuli, we assessed behavioral responses in ASIC3-/- and ASIC3+/+ mice after induction of carrageenan muscle inflammation. ASIC3-/- mice did not develop cutaneous mechanical hyperalgesia after muscle inflammation when compared to ASIC3+/+ mice; heat hyperalgesia developed similarly between groups. We then tested if the phenotype could be rescued in ASIC3-/- mice by using a recombinant herpes virus vector to express ASIC3 in skin (where testing occurred) or muscle (where inflammation occurred). Infection of mouse DRG neurons with ASIC3-encoding virus resulted in functional expression of ASICs. Injection of ASIC3-encoding virus into muscle or skin of ASIC3-/- mice resulted in ASIC3 mRNA in DRG and protein expression in DRG and the peripheral injection site. Injection of ASIC3-encoding virus into muscle, but not skin, resulted in development of mechanical hyperalgesia similar to that observed in ASIC3+/+ mice. Thus, ASIC3 in primary afferent fibers innervating muscle is critical to development of hyperalgesia that results from muscle insult. IS - 1-2 JF - Pain SN - 1872-6623 EP - 112 TI - ASIC3 in muscle mediates mechanical, but not heat, hyperalgesia associated with muscle inflammation. VL - 129 SP - 102 KW - asic3 KW - heat_hyperalgesia KW - mechanical_hyperalgesia KW - muscle_inflammation AU - Sluka, KA AU - Radhakrishnan, R AU - Benson, CJ AU - Eshcol, JO AU - Price, MP AU - Babinski, K AU - Audette, KM AU - Yeomans, DC AU - Wilson, SP PY - 2007/05// UR - http://dx.doi.org/10.1016/j.pain.2006.09.038 ER -