Serotonin stimulates endotoxin translocation via 5-HT3 receptors in the rat ileum Export

@article{citeulike:3846825, abstract = {Because few previous studies have investigated the mechanisms of endotoxin translocation induced by intestinal obstruction, we aimed to clarify whether or not serotonin [5-hydroxytryptamine (5-HT)], which is released from enterochromaffin (EC) cells, is responsible for alterations of the mucosal permeability to endotoxin and to identify the 5-HT receptor subtypes that mediate this action. FITC-labeled LPS (FITC-LPS) was injected into the ileum of rats, and the FITC-LPS level in the superior mesenteric vein was subsequently measured by using a fluorescence spectrophotometer. To measure the 5-HT release induced by high intraluminal pressure, ex vivo preparation of vascularly and luminally perfused rat ileum was used. Results demonstrated that elevated intraluminal pressure stimulates the translocation of FITC-LPS and the release of 5-HT from the EC cells into the intestinal lumen but not into the portal circulation. This FITC-LPS translocation, which was stimulated by exogenously applied 5-HT in the lumen and the jugular vein, was inhibited by 5-HT3 receptor antagonist administration both intaluminally and intravenously. The stimulatory effect of elevated intraluminal pressure on the translocation of FITC-LPS was inhibited by the intraluminal and intravenous administration of 5-HT3 receptor antagonist. These results suggest that 5-HT released from EC cells may be involved in the translocation of FITC-LPS induced by elevated intraluminal pressure and that this effect is mediated by 5-HT3 receptors that may be located in the intestinal epithelium. 10.1152/ajpgi.00376.2002}, author = {Yamada, Tadanori and Inui, Akio and Hayashi, Naoki and Fujimura, Masaki and Fujimiya, Mineko}, citeulike-article-id = {3846825}, citeulike-linkout-0 = {http://dx.doi.org/10.1152/ajpgi.00376.2002}, citeulike-linkout-1 = {http://ajpgi.physiology.org/cgi/content/abstract/284/5/G782}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/12684210}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=12684210}, day = {1}, doi = {10.1152/ajpgi.00376.2002}, journal = {Am J Physiol Gastrointest Liver Physiol}, keywords = {5-ht3\_receptors, endotoxin, serotonin}, month = {May}, number = {5}, pages = {G782--788}, posted-at = {2009-01-04 12:07:32}, priority = {3}, title = {Serotonin stimulates endotoxin translocation via 5-HT3 receptors in the rat ileum}, url = {http://dx.doi.org/10.1152/ajpgi.00376.2002}, volume = {284}, year = {2003} }

TY - JOUR ID - citeulike:3846825 L3 - citeulike-article-id:3846825 N2 - Because few previous studies have investigated the mechanisms of endotoxin translocation induced by intestinal obstruction, we aimed to clarify whether or not serotonin [5-hydroxytryptamine (5-HT)], which is released from enterochromaffin (EC) cells, is responsible for alterations of the mucosal permeability to endotoxin and to identify the 5-HT receptor subtypes that mediate this action. FITC-labeled LPS (FITC-LPS) was injected into the ileum of rats, and the FITC-LPS level in the superior mesenteric vein was subsequently measured by using a fluorescence spectrophotometer. To measure the 5-HT release induced by high intraluminal pressure, ex vivo preparation of vascularly and luminally perfused rat ileum was used. Results demonstrated that elevated intraluminal pressure stimulates the translocation of FITC-LPS and the release of 5-HT from the EC cells into the intestinal lumen but not into the portal circulation. This FITC-LPS translocation, which was stimulated by exogenously applied 5-HT in the lumen and the jugular vein, was inhibited by 5-HT3 receptor antagonist administration both intaluminally and intravenously. The stimulatory effect of elevated intraluminal pressure on the translocation of FITC-LPS was inhibited by the intraluminal and intravenous administration of 5-HT3 receptor antagonist. These results suggest that 5-HT released from EC cells may be involved in the translocation of FITC-LPS induced by elevated intraluminal pressure and that this effect is mediated by 5-HT3 receptors that may be located in the intestinal epithelium. 10.1152/ajpgi.00376.2002 IS - 5 JF - Am J Physiol Gastrointest Liver Physiol EP - 788 TI - Serotonin stimulates endotoxin translocation via 5-HT3 receptors in the rat ileum VL - 284 SP - G782 KW - 5-ht3_receptors KW - endotoxin KW - serotonin AU - Yamada, Tadanori AU - Inui, Akio AU - Hayashi, Naoki AU - Fujimura, Masaki AU - Fujimiya, Mineko PY - 2003/05/01/ UR - http://dx.doi.org/10.1152/ajpgi.00376.2002 ER -