Hesperidin inhibited acetaldehyde-induced matrix metalloproteinase-9 gene expression in human hepatocellular carcinoma cells. Export

@article{citeulike:3906412, abstract = {Previous studies have revealed that acetaldehyde-induced cell invasion and matrix metalloproteinase-9 (MMP-9) activation and are directly involved in hepatic tumorigenesis and metastasis. Acetaldehyde is an important substance for tumor regression. We designed this study to aid in the development of powerful anti-cancer drugs with specific tumor regression and anti-metastatic potentials. Optimal drugs should possess both specific MMP-9 enzyme and gene transcriptional activities at the molecular level. Hesperidin, a flavonoid present in fruits and vegetables, possess anti-inflammatory and chemopreventive activities. Hesperidin suppressed acetaldehyde-induced cell invasion and inhibited the secreted and cytosolic MMP-9 forms in HepG2 cells with acetaldehyde. Hesperidin suppressed acetaldehyde-induced MMP-9 expression through the inhibition of nuclear factor-kappaB (NF-kappaB) and AP-1, and suppressed acetaldehyde-stimulated NF-kappaB translocation into the nucleus through IkappaB inhibitory signaling pathways. Hesperidin also inhibited acetaldehyde-induced AP-1 activity by the inhibitory phosphorylation of p38 kinase and c-Jun N-terminal kinase (JNK) signaling pathways. Results from our study revealed that hesperidin suppressed both acetaldehyde-activated NF-kappaB and activator protein 1 (AP-1) activity by IkappaB, JNK, and p38 signaling pathways. This resulted in the reduction of MMP-9 expression, secretion, and hepatocarcinoma cellular invasion. Our result confirmed the therapeutic potential of hesperidin an anti-metastatic and its involvement in the acetaldehyde-induced cell invasiveness of hepatocellular carcinoma in alcoholic patients.}, author = {Yeh, Ming-Hsien H. and Kao, Shung-Te T. and Hung, Che-Ming M. and Liu, Ching-Ju J. and Lee, Kuo-Hua H. and Yeh, Chia-Chou C.}, citeulike-article-id = {3906412}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.toxlet.2008.11.018}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19110045}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19110045}, day = {6}, doi = {10.1016/j.toxlet.2008.11.018}, issn = {0378-4274}, journal = {Toxicology letters}, keywords = {acetyaldehyde, hesperidin, matrix\_metalloproteinase-9, mmp-9}, month = {December}, posted-at = {2009-01-18 21:05:04}, priority = {3}, title = {Hesperidin inhibited acetaldehyde-induced matrix metalloproteinase-9 gene expression in human hepatocellular carcinoma cells.}, url = {http://dx.doi.org/10.1016/j.toxlet.2008.11.018}, year = {2008} }

TY - JOUR ID - citeulike:3906412 L3 - citeulike-article-id:3906412 N2 - Previous studies have revealed that acetaldehyde-induced cell invasion and matrix metalloproteinase-9 (MMP-9) activation and are directly involved in hepatic tumorigenesis and metastasis. Acetaldehyde is an important substance for tumor regression. We designed this study to aid in the development of powerful anti-cancer drugs with specific tumor regression and anti-metastatic potentials. Optimal drugs should possess both specific MMP-9 enzyme and gene transcriptional activities at the molecular level. Hesperidin, a flavonoid present in fruits and vegetables, possess anti-inflammatory and chemopreventive activities. Hesperidin suppressed acetaldehyde-induced cell invasion and inhibited the secreted and cytosolic MMP-9 forms in HepG2 cells with acetaldehyde. Hesperidin suppressed acetaldehyde-induced MMP-9 expression through the inhibition of nuclear factor-kappaB (NF-kappaB) and AP-1, and suppressed acetaldehyde-stimulated NF-kappaB translocation into the nucleus through IkappaB inhibitory signaling pathways. Hesperidin also inhibited acetaldehyde-induced AP-1 activity by the inhibitory phosphorylation of p38 kinase and c-Jun N-terminal kinase (JNK) signaling pathways. Results from our study revealed that hesperidin suppressed both acetaldehyde-activated NF-kappaB and activator protein 1 (AP-1) activity by IkappaB, JNK, and p38 signaling pathways. This resulted in the reduction of MMP-9 expression, secretion, and hepatocarcinoma cellular invasion. Our result confirmed the therapeutic potential of hesperidin an anti-metastatic and its involvement in the acetaldehyde-induced cell invasiveness of hepatocellular carcinoma in alcoholic patients. JF - Toxicology letters SN - 0378-4274 TI - Hesperidin inhibited acetaldehyde-induced matrix metalloproteinase-9 gene expression in human hepatocellular carcinoma cells. KW - acetyaldehyde KW - hesperidin KW - matrix_metalloproteinase-9 KW - mmp-9 AU - Yeh, Ming-Hsien AU - Kao, Shung-Te AU - Hung, Che-Ming AU - Liu, Ching-Ju AU - Lee, Kuo-Hua AU - Yeh, Chia-Chou PY - 2008/12/06/ UR - http://dx.doi.org/10.1016/j.toxlet.2008.11.018 ER -