3-Hydroxyanthranilic acid, one of l-tryptophan metabolites, inhibits monocyte chemoattractant protein-1 secretion and vascular cell adhesion molecule-1 expression via heme oxygenase-1 induction in human umbilical vein endothelial cells Export

@article{citeulike:4229321, abstract = {Heme oxygenase (HO)-1 is important in the vascular system, and its genetic or pharmacological induction in endothelium would be effective for the prevention and treatment of atherosclerosis. The naturally occurring antioxidant 3-hydroxyanthranilic acid (HA), one of l -tryptophan metabolites formed in vivo along the metabolic route known as the kynurenine pathway during inflammation or infection, was found to induce HO-1 expression and to stimulate nuclear translocation of NF-E2 related factor 2 (Nrf2) in human umbilical vein endothelial cells (HUVECs). Pre-treatment with HA inhibited the secretion of monocyte chemoattractant protein (MCP)-1, the expression of vascular cell adhesion molecule (VCAM)-1 and the activation of transcriptional nuclear factor (NF)-κB in HUVECs stimulated with tumor necrosis factor-α, the major pro-inflammatory cytokine causing endothelial inflammation. Interestingly, the observed anti-inflammatory effects of HA were mimicked by a HO-1 inducer, cobalt protoporphyrin, and bilirubin, one of HO-1 enzymatic products, but abolished in the presence of a HO-1 inhibitor, tin protoporphyrin. Based on our findings, we suggest that Nrf2-dependent HO-1 expression induced by HA inhibits MCP-1 secretion, VCAM-1 expression and NF-κB activation associated with vascular injury and inflammation in atherosclerosis.}, author = {Pae, H. and Oh, G. and Lee, B. and Rim, J. and Kim, Y. and Chung, H.}, citeulike-article-id = {4229321}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.atherosclerosis.2005.09.010}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0021915005005964}, doi = {10.1016/j.atherosclerosis.2005.09.010}, issn = {00219150}, journal = {Atherosclerosis}, keywords = {3-hydroxyanthranilic\_acid, 5-ht, endothelial\_cells, ho-1, mcp-1, vcam-1}, month = {August}, number = {2}, pages = {274--284}, posted-at = {2009-03-27 10:16:43}, priority = {3}, title = {3-Hydroxyanthranilic acid, one of l-tryptophan metabolites, inhibits monocyte chemoattractant protein-1 secretion and vascular cell adhesion molecule-1 expression via heme oxygenase-1 induction in human umbilical vein endothelial cells}, url = {http://dx.doi.org/10.1016/j.atherosclerosis.2005.09.010}, volume = {187}, year = {2006} }

TY - JOUR ID - citeulike:4229321 L3 - citeulike-article-id:4229321 N2 - Heme oxygenase (HO)-1 is important in the vascular system, and its genetic or pharmacological induction in endothelium would be effective for the prevention and treatment of atherosclerosis. The naturally occurring antioxidant 3-hydroxyanthranilic acid (HA), one of l -tryptophan metabolites formed in vivo along the metabolic route known as the kynurenine pathway during inflammation or infection, was found to induce HO-1 expression and to stimulate nuclear translocation of NF-E2 related factor 2 (Nrf2) in human umbilical vein endothelial cells (HUVECs). Pre-treatment with HA inhibited the secretion of monocyte chemoattractant protein (MCP)-1, the expression of vascular cell adhesion molecule (VCAM)-1 and the activation of transcriptional nuclear factor (NF)-κB in HUVECs stimulated with tumor necrosis factor-α, the major pro-inflammatory cytokine causing endothelial inflammation. Interestingly, the observed anti-inflammatory effects of HA were mimicked by a HO-1 inducer, cobalt protoporphyrin, and bilirubin, one of HO-1 enzymatic products, but abolished in the presence of a HO-1 inhibitor, tin protoporphyrin. Based on our findings, we suggest that Nrf2-dependent HO-1 expression induced by HA inhibits MCP-1 secretion, VCAM-1 expression and NF-κB activation associated with vascular injury and inflammation in atherosclerosis. IS - 2 JF - Atherosclerosis SN - 00219150 EP - 284 TI - 3-Hydroxyanthranilic acid, one of l-tryptophan metabolites, inhibits monocyte chemoattractant protein-1 secretion and vascular cell adhesion molecule-1 expression via heme oxygenase-1 induction in human umbilical vein endothelial cells VL - 187 SP - 274 KW - 3-hydroxyanthranilic_acid KW - 5-ht KW - endothelial_cells KW - ho-1 KW - mcp-1 KW - vcam-1 AU - Pae, H AU - Oh, G AU - Lee, B AU - Rim, J AU - Kim, Y AU - Chung, H PY - 2006/08// UR - http://dx.doi.org/10.1016/j.atherosclerosis.2005.09.010 ER -