Expression of Interleukin-6 Is Greater in Preadipocytes than in Adipocytes of 3T3-L1 Cells and C57BL/6J and ob/ob Mice Export

@article{citeulike:4635310, abstract = {Inflammation plays a major role in the development of chronic diseases such as cardiovascular disease and Type 2 diabetes. Further, it was demonstrated that obese animals and humans have significantly higher levels of circulating proinflammatory cytokines, such as interleukin-6 (IL-6). The aim of this study was to determine whether adipose tissue could be a major source of circulating IL-6 in leptin-deficient obese (ob/ob) mice by comparing the expression of IL-6 in different tissues of ob/ob mice. Our secondary goal was to determine whether preadipocytes are the source of adipose tissue IL-6. The ob/ob mice had higher levels of plasma IL-6 (P < 0.05) and adipose tissue IL-6 mRNA (P < 0.05) compared with lean mice. Interestingly, IL-6 mRNA levels of liver and spleen were not different between ob/ob and lean mice, whereas adipose tissue IL-6 mRNA levels were higher in the ob/ob mice compared with lean mice (P < 0.05). In addition, we showed that IL-6 secretion from the adipose tissue stromal vascular fraction cells was higher than that from fully differentiated adipocytes (P < 0.001). We further demonstrated that 3T3-L1 preadipocytes had significantly higher levels of lipopolysaccharide (LPS)-stimulated IL-6 mRNA and IL-6 secretion than differentiated 3T3-L1 adipocytes. Taken together, these data suggest that adipose tissue and preadipocytes from the adipose tissue stromal vascular fraction may contribute significantly to the increased plasma IL-6 levels in ob/ob mice.}, author = {Harkins, Janette M. and Moustaid-Moussa, Naima and Chung, Yong-Joo and Penner, Kristen M. and Pestka, James J. and North, Colin M. and Claycombe, Kate J.}, citeulike-article-id = {4635310}, citeulike-linkout-0 = {http://jn.nutrition.org/cgi/content/abstract/134/10/2673}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15465765}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15465765}, day = {1}, journal = {J. Nutr.}, keywords = {il-6, preadipocytes}, month = {October}, number = {10}, pages = {2673--2677}, posted-at = {2009-05-26 11:51:40}, priority = {3}, title = {Expression of Interleukin-6 Is Greater in Preadipocytes than in Adipocytes of 3T3-L1 Cells and C57BL/6J and ob/ob Mice}, url = {http://jn.nutrition.org/cgi/content/abstract/134/10/2673}, volume = {134}, year = {2004} }

TY - JOUR ID - citeulike:4635310 L3 - citeulike-article-id:4635310 N2 - Inflammation plays a major role in the development of chronic diseases such as cardiovascular disease and Type 2 diabetes. Further, it was demonstrated that obese animals and humans have significantly higher levels of circulating proinflammatory cytokines, such as interleukin-6 (IL-6). The aim of this study was to determine whether adipose tissue could be a major source of circulating IL-6 in leptin-deficient obese (ob/ob) mice by comparing the expression of IL-6 in different tissues of ob/ob mice. Our secondary goal was to determine whether preadipocytes are the source of adipose tissue IL-6. The ob/ob mice had higher levels of plasma IL-6 (P < 0.05) and adipose tissue IL-6 mRNA (P < 0.05) compared with lean mice. Interestingly, IL-6 mRNA levels of liver and spleen were not different between ob/ob and lean mice, whereas adipose tissue IL-6 mRNA levels were higher in the ob/ob mice compared with lean mice (P < 0.05). In addition, we showed that IL-6 secretion from the adipose tissue stromal vascular fraction cells was higher than that from fully differentiated adipocytes (P < 0.001). We further demonstrated that 3T3-L1 preadipocytes had significantly higher levels of lipopolysaccharide (LPS)-stimulated IL-6 mRNA and IL-6 secretion than differentiated 3T3-L1 adipocytes. Taken together, these data suggest that adipose tissue and preadipocytes from the adipose tissue stromal vascular fraction may contribute significantly to the increased plasma IL-6 levels in ob/ob mice. IS - 10 JF - J. Nutr. EP - 2677 TI - Expression of Interleukin-6 Is Greater in Preadipocytes than in Adipocytes of 3T3-L1 Cells and C57BL/6J and ob/ob Mice VL - 134 SP - 2673 KW - il-6 KW - preadipocytes AU - Harkins, Janette AU - Moustaid-Moussa, Naima AU - Chung, Yong-Joo AU - Penner, Kristen AU - Pestka, James AU - North, Colin AU - Claycombe, Kate PY - 2004/10/01/ UR - http://jn.nutrition.org/cgi/content/abstract/134/10/2673 ER -