B cell receptor-mediated uptake of CD1d-restricted antigen augments antibody responses by recruiting invariant NKT cell help in vivo. Export

@article{citeulike:3041272, abstract = {Highly regulated activation of B cells is required for the production of specific antibodies necessary to provide protection from pathogen infection. This process is initiated by specific recognition of antigen through the B cell receptor (BCR), leading to early intracellular signaling followed by the late recruitment of T cell help. In this study we demonstrate that specific BCR uptake of CD1d-restricted antigens represents an effective means of enhancing invariant natural killer T (iNKT)-dependent B cell responses in vivo. This mechanism is effective over a wide range of antigen affinities but depends on exceeding a tightly regulated avidity threshold necessary for BCR-mediated internalization and CD1d-dependent presentation of particulate antigenic lipid. Subsequently, iNKT cells provide the help required for stimulating B cell proliferation and differentiation. iNKT-stimulated B cells develop within extrafollicular foci and mediate the production of high titers of specific IgM and early class-switched antibodies. Thus, we have demonstrated that in response to particulate antigenic lipids iNKT cells are recruited for the assistance of B cell activation, resulting in the enhancement of specific antibody responses. We propose that such a mechanism may operate to potentiate adaptive immune responses against pathogens in vivo.}, address = {Lymphocyte Interaction Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom.}, author = {Barral, P. and Eckl-Dorna, J. and Harwood, N. E. and De Santo, C. and Salio, M. and Illarionov, P. and Besra, G. S. and Cerundolo, V. and Batista, F. D.}, citeulike-article-id = {3041272}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0802968105}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18550831}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18550831}, day = {17}, doi = {10.1073/pnas.0802968105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {antibody, b-cell, nkt\_cell}, month = {June}, number = {24}, pages = {8345--8350}, posted-at = {2008-07-24 22:12:22}, priority = {2}, title = {B cell receptor-mediated uptake of CD1d-restricted antigen augments antibody responses by recruiting invariant NKT cell help in vivo.}, url = {http://dx.doi.org/10.1073/pnas.0802968105}, volume = {105}, year = {2008} }

TY - JOUR ID - citeulike:3041272 L3 - citeulike-article-id:3041272 N2 - Highly regulated activation of B cells is required for the production of specific antibodies necessary to provide protection from pathogen infection. This process is initiated by specific recognition of antigen through the B cell receptor (BCR), leading to early intracellular signaling followed by the late recruitment of T cell help. In this study we demonstrate that specific BCR uptake of CD1d-restricted antigens represents an effective means of enhancing invariant natural killer T (iNKT)-dependent B cell responses in vivo. This mechanism is effective over a wide range of antigen affinities but depends on exceeding a tightly regulated avidity threshold necessary for BCR-mediated internalization and CD1d-dependent presentation of particulate antigenic lipid. Subsequently, iNKT cells provide the help required for stimulating B cell proliferation and differentiation. iNKT-stimulated B cells develop within extrafollicular foci and mediate the production of high titers of specific IgM and early class-switched antibodies. Thus, we have demonstrated that in response to particulate antigenic lipids iNKT cells are recruited for the assistance of B cell activation, resulting in the enhancement of specific antibody responses. We propose that such a mechanism may operate to potentiate adaptive immune responses against pathogens in vivo. IS - 24 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 1091-6490 AD - Lymphocyte Interaction Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3PX, United Kingdom. EP - 8350 TI - B cell receptor-mediated uptake of CD1d-restricted antigen augments antibody responses by recruiting invariant NKT cell help in vivo. VL - 105 SP - 8345 KW - antibody KW - b-cell KW - nkt_cell AU - Barral, P AU - Eckl-Dorna, J AU - Harwood, NE AU - De Santo, C AU - Salio, M AU - Illarionov, P AU - Besra, GS AU - Cerundolo, V AU - Batista, FD PY - 2008/06/17/ UR - http://dx.doi.org/10.1073/pnas.0802968105 ER -