Immunophenotypic characterization of peripheral T lymphocytes in Mycobacterium tuberculosis infection and disease.

@article{citeulike:3043975, abstract = {The cellular immune response probably plays a pivotal role in determining the clinical outcome after exposure to Mycobacterium tuberculosis. We used multi-parameter flow-cytometry to evaluate the distribution of T-lymphocyte subsets during infection and disease caused by M. tuberculosis. Samples were obtained from 71 volunteers to identify the T CD4+ and CD8+ lymphocyte numbers, and the activation plus memory/na\"{i}ve phenotypes, as defined by CD38, HLA-DR, CD45RA and CD27 markers. Subjects were divided into 18 healthy volunteers without detectable reaction to purified protein derivative (PPD-), 18 health care workers with a recent conversion to PPD, 20 patients with active pulmonary tuberculosis (TBC) and 15 patients with treated TBC at 6 months of therapy. By multiple-comparison analyses, the T CD4+ lymphocyte number of the TBC group was lower than the PPD- group (P < 0.05). This difference was apparently lost after treatment. The higher and the lower number of na\"{i}ve T CD4+ cells was observed in the PPD- and TBC group, respectively. CD8+ T lymphocytes were also statistically different among the four groups (P = 0.0002), lower in the TBC group (P < 0.05). CD8+ T lymphocyte activation was evaluated by the CD38 and HLA-DR surface expression. The percentage distribution of these markers was statistically different between the four groups (P = 0.0055). TBC patients had a higher percentage of CD38+ cells and mean fluorescence index, suggesting an overall increase of cell activation. These results suggest that peripheral T lymphocytes reflect cellular activation during TBC, along with possible redistribution of na\"{i}ve, memory/effector and late differentiated memory/effector phenotypes in the peripheral blood after infection and disease caused by M. tuberculosis.}, address = {Infectious Diseases Discipline and Immunization Center, Federal University of S\~{a}o Paulo, S\~{a}o Paulo, Brazil.}, author = {Rodrigues, D. S. and Medeiros, E. A. and Weckx, L. Y. and Bonnez, W. and Salom\~{a}o, R. and Kallas, E. G. }, citeulike-article-id = {3043975}, issn = {0009-9104}, journal = {Clinical and experimental immunology}, keywords = {cd4\_t\_cell, cd8\_t\_cell, immunophenotype, tania\_tb\_paper, tuberculosis}, month = {April}, number = {1}, pages = {149--154}, posted-at = {2008-07-26 02:09:20}, priority = {2}, title = {Immunophenotypic characterization of peripheral T lymphocytes in Mycobacterium tuberculosis infection and disease.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11982602}, volume = {128}, year = {2002} }

TY - JOUR ID - citeulike:3043975 L3 - citeulike-article-id:3043975 TI - Immunophenotypic characterization of peripheral T lymphocytes in Mycobacterium tuberculosis infection and disease. JF - Clinical and experimental immunology VL - 128 IS - 1 SP - 149 EP - 154 AD - Infectious Diseases Discipline and Immunization Center, Federal University of São Paulo, São Paulo, Brazil. SN - 0009-9104 N2 - The cellular immune response probably plays a pivotal role in determining the clinical outcome after exposure to Mycobacterium tuberculosis. We used multi-parameter flow-cytometry to evaluate the distribution of T-lymphocyte subsets during infection and disease caused by M. tuberculosis. Samples were obtained from 71 volunteers to identify the T CD4+ and CD8+ lymphocyte numbers, and the activation plus memory/naïve phenotypes, as defined by CD38, HLA-DR, CD45RA and CD27 markers. Subjects were divided into 18 healthy volunteers without detectable reaction to purified protein derivative (PPD-), 18 health care workers with a recent conversion to PPD, 20 patients with active pulmonary tuberculosis (TBC) and 15 patients with treated TBC at 6 months of therapy. By multiple-comparison analyses, the T CD4+ lymphocyte number of the TBC group was lower than the PPD- group (P < 0.05). This difference was apparently lost after treatment. The higher and the lower number of naïve T CD4+ cells was observed in the PPD- and TBC group, respectively. CD8+ T lymphocytes were also statistically different among the four groups (P = 0.0002), lower in the TBC group (P < 0.05). CD8+ T lymphocyte activation was evaluated by the CD38 and HLA-DR surface expression. The percentage distribution of these markers was statistically different between the four groups (P = 0.0055). TBC patients had a higher percentage of CD38+ cells and mean fluorescence index, suggesting an overall increase of cell activation. These results suggest that peripheral T lymphocytes reflect cellular activation during TBC, along with possible redistribution of naïve, memory/effector and late differentiated memory/effector phenotypes in the peripheral blood after infection and disease caused by M. tuberculosis. KW - cd4_t_cell KW - cd8_t_cell KW - immunophenotype KW - tania_tb_paper KW - tuberculosis AU - Rodrigues, DS AU - Medeiros, EA AU - Weckx, LY AU - Bonnez, W AU - Salomão, R AU - Kallas, EG PY - 2002/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/11982602 ER -