Peripheral blood mononuclear cells immunophenotyping in pulmonary tuberculosis patients before and after treatment. Export

@article{citeulike:3044158, abstract = {Tuberculosis (TB) is a lung disease caused by Mycobacterium tuberculosis. The interaction between the bacillus and the host may lead to a protective cellular immune response. In the present study, we propose the "in vitro" evaluation of this cellular immune response in patients with tuberculosis before and after chemotherapic treatment. Eleven patients with TB and 9 asymptomatic subjects with tuberculin skin test negative (TST-) (purified protein derivative (PPD) <or=10 mm) were evaluated. The peripheral lymphocytes of the subjects were analyzed utilizing the following surface markers: CD3(+), CD4(+), CD8(+), CD19(+), CD25(+), CD56(+), CD14(+), CD16(+) and HLA-DR(+). At the end of the treatment, symptomatic patients presented a significant predominance (P <0.05) of CD4(+) lymphocytes, a significant decrease (P <0.05) in activated CD8(+) T cells and a significant increase (P <0.05) in the marker CD19(+). A predominance of mature NK cells and a significant decrease (P <0.05) in NKT cells were observed. Also observed was a trend toward decrease in immunoregulatory T cells and a predominance of pro-inflammatory macrophages. TST- subjects presented a predominance of CD4(+) over CD8(+) and predominance of CD19(+) and of mature NK cells in comparison to the group of patients, both before and after treatment. Thus, several cell types, such as CD4(+) and CD8(+) T cells, NK cells and their subpopulations, NKT cells and pro-inflammatory macrophages could act in a synergic way to control the growth and multiplication of M. tuberculosis.}, address = {Departamento de An\'{a}lises Cl\'{\i}nicas e Toxicol\'{o}gicas, Faculdade de Farm\'{a}cia da UFMG, Belo Horizonte, MG, Brasil.}, author = {Barcelos, W. and Martins-Filho, O. A. and Guimar\~{a}es, T. M. and Oliveira, M. H. and Sp\'{\i}ndola-de-Miranda, S. and Carvalho, B. N. and Toledo, V. d. e. . P.}, citeulike-article-id = {3044158}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/16924144}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=16924144}, issn = {0385-5600}, journal = {Microbiology and immunology}, keywords = {b-cell, cd38, cd4\_t\_cell, cd8\_t\_cell, immunophenotype, tuberculosis}, number = {8}, pages = {597--605}, posted-at = {2008-07-26 03:11:26}, priority = {2}, title = {Peripheral blood mononuclear cells immunophenotyping in pulmonary tuberculosis patients before and after treatment.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/16924144}, volume = {50}, year = {2006} }

TY - JOUR ID - citeulike:3044158 L3 - citeulike-article-id:3044158 N2 - Tuberculosis (TB) is a lung disease caused by Mycobacterium tuberculosis. The interaction between the bacillus and the host may lead to a protective cellular immune response. In the present study, we propose the "in vitro" evaluation of this cellular immune response in patients with tuberculosis before and after chemotherapic treatment. Eleven patients with TB and 9 asymptomatic subjects with tuberculin skin test negative (TST-) (purified protein derivative (PPD) <or=10 mm) were evaluated. The peripheral lymphocytes of the subjects were analyzed utilizing the following surface markers: CD3(+), CD4(+), CD8(+), CD19(+), CD25(+), CD56(+), CD14(+), CD16(+) and HLA-DR(+). At the end of the treatment, symptomatic patients presented a significant predominance (P <0.05) of CD4(+) lymphocytes, a significant decrease (P <0.05) in activated CD8(+) T cells and a significant increase (P <0.05) in the marker CD19(+). A predominance of mature NK cells and a significant decrease (P <0.05) in NKT cells were observed. Also observed was a trend toward decrease in immunoregulatory T cells and a predominance of pro-inflammatory macrophages. TST- subjects presented a predominance of CD4(+) over CD8(+) and predominance of CD19(+) and of mature NK cells in comparison to the group of patients, both before and after treatment. Thus, several cell types, such as CD4(+) and CD8(+) T cells, NK cells and their subpopulations, NKT cells and pro-inflammatory macrophages could act in a synergic way to control the growth and multiplication of M. tuberculosis. IS - 8 JF - Microbiology and immunology SN - 0385-5600 AD - Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia da UFMG, Belo Horizonte, MG, Brasil. EP - 605 TI - Peripheral blood mononuclear cells immunophenotyping in pulmonary tuberculosis patients before and after treatment. VL - 50 SP - 597 KW - b-cell KW - cd38 KW - cd4_t_cell KW - cd8_t_cell KW - immunophenotype KW - tuberculosis AU - Barcelos, W AU - Martins-Filho, OA AU - Guimarães, TM AU - Oliveira, MH AU - Spíndola-de-Miranda, S AU - Carvalho, BN AU - Toledo, Vde P PY - 2006/// UR - http://view.ncbi.nlm.nih.gov/pubmed/16924144 ER -