Treponema pallidum elicits innate and adaptive cellular immune responses in skin and blood during secondary syphilis: a flow-cytometric analysis. Export

@article{citeulike:3044181, abstract = {BACKGROUND: Syphilis is caused by the spirochetal pathogen Treponema pallidum. The local and systemic cellular immune responses elicited by the bacterium have not been well studied in humans. METHODS: We used multiparameter flow cytometry to characterize leukocyte immunophenotypes in skin and peripheral blood from 23 patients with secondary syphilis and 5 healthy control subjects recruited in Cali, Colombia. Dermal leukocytes were obtained from fluid aspirated from epidermal suction blisters raised over secondary syphilis skin lesions. RESULTS: Compared with peripheral blood (PB), blister fluids (BFs) were enriched for CD4(+) and CD8(+) T cells, activated monocytes/macrophages, and CD11c(+) monocytoid and CD11c(-) plasmacytoid dendritic cells (mDCs and pDCs, respectively). Nearly all mDCs in BFs expressed the human immunodeficiency virus (HIV) coreceptors CCR5 and DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and high levels of human leukocyte antigen (HLA)-DR. Dermal pDCs expressed both HIV coreceptors without increases in HLA-DR intensity. Compared with normal blood, circulating mDCs in patients with syphilis expressed higher levels of both CCR5 and DC-SIGN, whereas circulating pDCs in patients expressed only higher levels of DC-SIGN. Most dermal T cells were CCR5(+) and displayed a memory (CD27(+)/CD45RO(+)) or memory/effector (CD27(-)/CD45RO(+)) immunophenotype. A corresponding shift toward memory and memory/effector immunophenotype was clearly discernible among circulating CD4(+) T cells. Compared with PB from control subjects, a larger percentage of CD4(+) T cells in PB from patients with syphilis expressed the activation markers CD69 and CD38. CONCLUSIONS: During secondary syphilis, T. pallidum simultaneously elicits local and systemic innate and adaptive immune responses that may set the stage for the bidirectional transmission of HIV.}, address = {Department of Pediatrics, Connecticut Children's Medical Center, Division of Pediatric Infectious Diseases, Hartford, CT, 06106, USA. jsalaza@ccmckids.org}, author = {Salazar, J. C. and Cruz, A. R. and Pope, C. D. and Valderrama, L. and Trujillo, R. and Saravia, N. G. and Radolf, J. D.}, citeulike-article-id = {3044181}, citeulike-linkout-0 = {http://dx.doi.org/10.1086/511822}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17299719}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17299719}, doi = {10.1086/511822}, issn = {0022-1899}, journal = {The Journal of infectious diseases}, keywords = {immunology, innate\_immunol, std, syphilis, tania\_tb\_paper}, month = {March}, number = {6}, pages = {879--887}, posted-at = {2008-07-26 03:52:16}, priority = {2}, title = {Treponema pallidum elicits innate and adaptive cellular immune responses in skin and blood during secondary syphilis: a flow-cytometric analysis.}, url = {http://dx.doi.org/10.1086/511822}, volume = {195}, year = {2007} }

TY - JOUR ID - citeulike:3044181 L3 - citeulike-article-id:3044181 N2 - BACKGROUND: Syphilis is caused by the spirochetal pathogen Treponema pallidum. The local and systemic cellular immune responses elicited by the bacterium have not been well studied in humans. METHODS: We used multiparameter flow cytometry to characterize leukocyte immunophenotypes in skin and peripheral blood from 23 patients with secondary syphilis and 5 healthy control subjects recruited in Cali, Colombia. Dermal leukocytes were obtained from fluid aspirated from epidermal suction blisters raised over secondary syphilis skin lesions. RESULTS: Compared with peripheral blood (PB), blister fluids (BFs) were enriched for CD4(+) and CD8(+) T cells, activated monocytes/macrophages, and CD11c(+) monocytoid and CD11c(-) plasmacytoid dendritic cells (mDCs and pDCs, respectively). Nearly all mDCs in BFs expressed the human immunodeficiency virus (HIV) coreceptors CCR5 and DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) and high levels of human leukocyte antigen (HLA)-DR. Dermal pDCs expressed both HIV coreceptors without increases in HLA-DR intensity. Compared with normal blood, circulating mDCs in patients with syphilis expressed higher levels of both CCR5 and DC-SIGN, whereas circulating pDCs in patients expressed only higher levels of DC-SIGN. Most dermal T cells were CCR5(+) and displayed a memory (CD27(+)/CD45RO(+)) or memory/effector (CD27(-)/CD45RO(+)) immunophenotype. A corresponding shift toward memory and memory/effector immunophenotype was clearly discernible among circulating CD4(+) T cells. Compared with PB from control subjects, a larger percentage of CD4(+) T cells in PB from patients with syphilis expressed the activation markers CD69 and CD38. CONCLUSIONS: During secondary syphilis, T. pallidum simultaneously elicits local and systemic innate and adaptive immune responses that may set the stage for the bidirectional transmission of HIV. IS - 6 JF - The Journal of infectious diseases SN - 0022-1899 AD - Department of Pediatrics, Connecticut Children's Medical Center, Division of Pediatric Infectious Diseases, Hartford, CT, 06106, USA. jsalaza@ccmckids.org EP - 887 TI - Treponema pallidum elicits innate and adaptive cellular immune responses in skin and blood during secondary syphilis: a flow-cytometric analysis. VL - 195 SP - 879 KW - immunology KW - innate_immunol KW - std KW - syphilis KW - tania_tb_paper AU - Salazar, JC AU - Cruz, AR AU - Pope, CD AU - Valderrama, L AU - Trujillo, R AU - Saravia, NG AU - Radolf, JD PY - 2007/03/15/ UR - http://dx.doi.org/10.1086/511822 ER -