Increased Regulatory T Cells Correlate With CD8 T-Cell Impairment and Poor Survival in Hepatocellular Carcinoma Patients Export

@article{citeulike:3097046, abstract = {Background \& Aims Recent studies have suggested that CD4+CD25+ regulatory T cells (Treg) are increased and linked to compromised immune responses in patients with hepatocellular carcinoma (HCC). This study attempted to further characterize CD4+CD25+ forkhead/winged helix transcription factor (FoxP3)+ Treg in blood, tumor, and nontumor liver tissues of HCC patients, and to understand how the Treg affects immune responses and contributes to disease progression.Methods A total of 123 HCC patients with chronic hepatitis B virus (HBV) infection, 21 HBV-related liver cirrhosis (LC) patients, and 47 normal controls were enrolled randomly. Flow cytometric, immunohistochemical, and immunosuppressive assays were used for analyses of properties of Treg. Multivariate analysis of prognostic factors for overall survival was performed using the Cox proportional hazards model.Results Circulating CD4+CD25+FoxP3+ Treg frequency was increased significantly and correlated with disease progression in HCC patients. An abundant accumulation of Treg concurrent with significantly reduced infiltration of CD8+ T cells was found in tumor regions compared with nontumor regions. Expression of granzyme A, granzyme B, and perforin was decreased dramatically in tumor-infiltrating CD8+ T cells. Furthermore, Treg of HCC patients inhibited proliferation, activation, degranulation, and production of granzyme A, granzyme B, and perforin of CD8+ T cells induced by anti-CD3/CD28 antibodies. Importantly, an increased quantity of circulating Treg was associated with high mortality and reduced survival time of HCC patients.Conclusions Increased CD4+CD25+FoxP3+ Treg may impair the effector function of CD8+ T cells, promote disease progression, and represent both a potential prognostic marker and a therapeutic target for HBV-related HCC individuals.}, author = {Fu, Junliang and Xu, Dongping and Liu, Zhenwen and Shi, Ming and Zhao, Ping and Fu, Baoyun and Zhang, Zheng and Yang, Huiyin and Zhang, Hui and Zhou, Chunbao and Yao, Jinxia and Jin, Lei and Wang, Huifen and Yang, Yongping and Fu, Yang-Xing and Wang, Fu-Sheng}, citeulike-article-id = {3097046}, citeulike-linkout-0 = {http://dx.doi.org/10.1053/j.gastro.2007.03.102}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WFX-4NGRRSC-3/2/dbd280c4cf6ea11dea2fc3c1be9bb6b2}, doi = {10.1053/j.gastro.2007.03.102}, journal = {Gastroenterology}, keywords = {cd8\_t\_cell, hepatocellular\_carcinoma, treg}, month = {June}, number = {7}, pages = {2328--2339}, posted-at = {2008-08-07 22:28:40}, priority = {2}, title = {Increased Regulatory T Cells Correlate With CD8 T-Cell Impairment and Poor Survival in Hepatocellular Carcinoma Patients}, url = {http://dx.doi.org/10.1053/j.gastro.2007.03.102}, volume = {132}, year = {2007} }

TY - JOUR ID - citeulike:3097046 L3 - citeulike-article-id:3097046 N2 - Background & Aims Recent studies have suggested that CD4+CD25+ regulatory T cells (Treg) are increased and linked to compromised immune responses in patients with hepatocellular carcinoma (HCC). This study attempted to further characterize CD4+CD25+ forkhead/winged helix transcription factor (FoxP3)+ Treg in blood, tumor, and nontumor liver tissues of HCC patients, and to understand how the Treg affects immune responses and contributes to disease progression.Methods A total of 123 HCC patients with chronic hepatitis B virus (HBV) infection, 21 HBV-related liver cirrhosis (LC) patients, and 47 normal controls were enrolled randomly. Flow cytometric, immunohistochemical, and immunosuppressive assays were used for analyses of properties of Treg. Multivariate analysis of prognostic factors for overall survival was performed using the Cox proportional hazards model.Results Circulating CD4+CD25+FoxP3+ Treg frequency was increased significantly and correlated with disease progression in HCC patients. An abundant accumulation of Treg concurrent with significantly reduced infiltration of CD8+ T cells was found in tumor regions compared with nontumor regions. Expression of granzyme A, granzyme B, and perforin was decreased dramatically in tumor-infiltrating CD8+ T cells. Furthermore, Treg of HCC patients inhibited proliferation, activation, degranulation, and production of granzyme A, granzyme B, and perforin of CD8+ T cells induced by anti-CD3/CD28 antibodies. Importantly, an increased quantity of circulating Treg was associated with high mortality and reduced survival time of HCC patients.Conclusions Increased CD4+CD25+FoxP3+ Treg may impair the effector function of CD8+ T cells, promote disease progression, and represent both a potential prognostic marker and a therapeutic target for HBV-related HCC individuals. IS - 7 JF - Gastroenterology EP - 2339 TI - Increased Regulatory T Cells Correlate With CD8 T-Cell Impairment and Poor Survival in Hepatocellular Carcinoma Patients VL - 132 SP - 2328 KW - cd8_t_cell KW - hepatocellular_carcinoma KW - treg AU - Fu, Junliang AU - Xu, Dongping AU - Liu, Zhenwen AU - Shi, Ming AU - Zhao, Ping AU - Fu, Baoyun AU - Zhang, Zheng AU - Yang, Huiyin AU - Zhang, Hui AU - Zhou, Chunbao AU - Yao, Jinxia AU - Jin, Lei AU - Wang, Huifen AU - Yang, Yongping AU - Fu, Yang-Xing AU - Wang, Fu-Sheng PY - 2007/06// UR - http://dx.doi.org/10.1053/j.gastro.2007.03.102 ER -