Kinetic study of biosurfactant production by Bacillus subtilis LAMI005 grown in clarified cashew apple juice
In this work a low cost medium for the production of a biosurfactant by Bacillus subtilis LAMI005 and the kinetics of surfactin production considering the effect of initial substrate concentration were investigated. First, cashew apple juice supplementation for optimal production of biosurfactant by B. subtilis LAMI005 was studied. The medium formulated with clarified cashew apple juice and distilled water, supplemented with 1.0 g/L of (NH4)2SO4, proved to be the best among the nutrients evaluated. The crude biosurfactant had the ability to decrease the surface tension of water to 30 dyne/cm, with a critical micelle concentration (CMC) of 63.0 mg/L. Emulsification experiments indicated that this biosurfactant effectively emulsified kerosene (IE24 = 67%) and soybean oil (IE24 = 64%). Furthermore, the emulsion stability was always very high. It was shown by biochemical analysis, IR spectra, that there is no qualitative differences in the composition of the crude biosurfactant from a standard sample of surfactin from B. subtilis. âº Biosurfactant production by Bacillus subtilis LAMI005. âº Use of clarified cashew apple juice as low cost culture medium. âº Influence of medium supplementation on biosurfactant production. âº Critical micelle concentration (CMC) of surfactin produced by B. subtilis LAMI005 at this work ranged from 10.24 to 63 mg/L. âº Infrared spectrum of the B. subitilis LAMI005 biosurfactant indicates that surfactin was produced.