Economical production of poly(γ-glutamic acid) using untreated cane molasses and monosodium glutamate waste liquor by Bacillus subtilis NX-2

The production of poly(γ-glutamic acid) by Bacillus subtilis NX-2 from cane molasses and monosodium glutamate waste liquor (MGWL) was studied for the first time in this work. When batch fermentation was carried out with untreated molasses, 33.6 ± 0.37 g L−1 PGA was obtained with a productivity of 0.46 ± 0.006 g L−1 h−1. In order to minimize the substrate inhibition, fed-batch fermentation was performed with untreated or hydrolyzed molasses in 7.5 L bioreactor, giving 50.2 ± 0.53 and 51.1 ± 0.51 g L−1 of PGA at 96 h, respectively. Further studies were carried out by using MGWL as another carbon source, resulting in a PGA concentration of 52.1 ± 0.52 g L−1 with a productivity of 0.54 ± 0.003 g L−1 h−1. These results suggest that the low-cost cane molasses and MGWL can be used for the environmental-friendly and economical production of PGA by B. subtilis NX-2. ⺠Economical and high yield production of PGA used waste materials was realized. ⺠Untreated cane molasses was used as carbon source. ⺠MGWL was used by supplying glutamate and (NH4)2SO4. ⺠Half of the material cost could be saved by using these low-cost materials.