Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli. Export

@article{citeulike:3750462, abstract = {Broad-acting transcription factors (TFs) in bacteria form regulons. Here, we present a 4-step method to fully reconstruct the leucine-responsive protein (Lrp) regulon in Escherichia coli K-12 MG 1655 that regulates nitrogen metabolism. Step 1 is composed of obtaining high-resolution ChIP-chip data for Lrp, the RNA polymerase and expression profiles under multiple environmental conditions. We identified 138 unique and reproducible Lrp-binding regions and classified their binding state under different conditions. In the second step, the analysis of these data revealed 6 distinct regulatory modes for individual ORFs. In the third step, we used the functional assignment of the regulated ORFs to reconstruct 4 types of regulatory network motifs around the metabolites that are affected by the corresponding gene products. In the fourth step, we determined how leucine, as a signaling molecule, shifts the regulatory motifs for particular metabolites. The physiological structure that emerges shows the regulatory motifs for different amino acid fall into the traditional classification of amino acid families, thus elucidating the structure and physiological functions of the Lrp-regulon. The same procedure can be applied to other broad-acting TFs, opening the way to full bottom-up reconstruction of the transcriptional regulatory network in bacterial cells.}, author = {Cho, Byung-Kwan K. and Barrett, Christian L L. and Knight, Eric M M. and Park, Young Seoub S. and Palsson, Bernhard {\O} O.}, citeulike-article-id = {3750462}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0807227105}, citeulike-linkout-1 = {http://www.pnas.org/content/105/49/19462.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/105/49/19462.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/19052235}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=19052235}, day = {3}, doi = {10.1073/pnas.0807227105}, issn = {1091-6490}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {chipchip, chipseq, ecoli, gene-regulation, transcription-factors}, month = {December}, posted-at = {2009-03-05 19:33:07}, priority = {2}, title = {Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli.}, url = {http://dx.doi.org/10.1073/pnas.0807227105}, year = {2008} }

TY - JOUR ID - citeulike:3750462 L3 - citeulike-article-id:3750462 N2 - Broad-acting transcription factors (TFs) in bacteria form regulons. Here, we present a 4-step method to fully reconstruct the leucine-responsive protein (Lrp) regulon in Escherichia coli K-12 MG 1655 that regulates nitrogen metabolism. Step 1 is composed of obtaining high-resolution ChIP-chip data for Lrp, the RNA polymerase and expression profiles under multiple environmental conditions. We identified 138 unique and reproducible Lrp-binding regions and classified their binding state under different conditions. In the second step, the analysis of these data revealed 6 distinct regulatory modes for individual ORFs. In the third step, we used the functional assignment of the regulated ORFs to reconstruct 4 types of regulatory network motifs around the metabolites that are affected by the corresponding gene products. In the fourth step, we determined how leucine, as a signaling molecule, shifts the regulatory motifs for particular metabolites. The physiological structure that emerges shows the regulatory motifs for different amino acid fall into the traditional classification of amino acid families, thus elucidating the structure and physiological functions of the Lrp-regulon. The same procedure can be applied to other broad-acting TFs, opening the way to full bottom-up reconstruction of the transcriptional regulatory network in bacterial cells. JF - Proceedings of the National Academy of Sciences of the United States of America SN - 1091-6490 TI - Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli. KW - chipchip KW - chipseq KW - ecoli KW - gene-regulation KW - transcription-factors AU - Cho, Byung-Kwan AU - Barrett, Christian L AU - Knight, Eric M AU - Park, Young Seoub AU - Palsson, Bernhard Ø PY - 2008/12/03/ UR - http://dx.doi.org/10.1073/pnas.0807227105 ER -