Synergistic Hydrolysis of Carboxymethyl Cellulose and Acid-Swollen Cellulose by Two Endoglucanases (CelZ and CelY) from Erwinia chrysanthemi Export

@article{citeulike:3980208, abstract = {Erwinia chrysanthemi produces a battery of hydrolases and lyases which are very effective in the maceration of plant cell walls. Although two endoglucanases (CelZ and CelY; formerly EGZ and EGY) are produced, CelZ represents approximately 95\% of the total carboxymethyl cellulase activity. In this study, we have examined the effectiveness of CelY and CelZ alone and of combinations of both enzymes using carboxymethyl cellulose (CMC) and amorphous cellulose (acid-swollen cellulose) as substrates. Synergy was observed with both substrates. Maximal synergy (1.8-fold) was observed for combinations containing primarily CelZ; the ratio of enzyme activities produced was similar to those produced by cultures of E. chrysanthemi. CelY and CelZ were quite different in substrate preference. CelY was unable to hydrolyze soluble cellooligosaccharides (cellotetraose and cellopentaose) but hydrolyzed CMC to fragments averaging 10.7 glucosyl units. In contrast, CelZ readily hydrolyzed cellotetraose, cellopentaose, and amorphous cellulose to produce cellobiose and cellotriose as dominant products. CelZ hydrolyzed CMC to fragments averaging 3.6 glucosyl units. In combination, CelZ and CelY hydrolyzed CMC to products averaging 2.3 glucosyl units. Synergy did not require the simultaneous presence of both enzymes. Enzymatic modification of the substrate by CelY increased the rate and extent of hydrolysis by CelZ. Full synergy was retained by the sequential hydrolysis of CMC, provided CelY was used as the first enzyme. A general mechanism is proposed to explain the synergy between these two enzymes based primarily on differences in substrate preference. 10.1128/JB.182.20.5676-5682.2000}, author = {Zhou, Shengde and Ingram, Lonnie O.}, citeulike-article-id = {3980208}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JB.182.20.5676-5682.2000}, citeulike-linkout-1 = {http://jb.asm.org/cgi/content/abstract/182/20/5676?ijkey=fc206acae80c364b1dc5a560b7c84a60f9a5664f&keytype2=tf_ipsecsha}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/11004164}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=11004164}, day = {15}, doi = {10.1128/JB.182.20.5676-5682.2000}, journal = {J. Bacteriol.}, keywords = {cellulase, cellulose, cmc, endoglucanase}, month = {October}, number = {20}, pages = {5676--5682}, posted-at = {2009-01-30 02:49:38}, priority = {2}, title = {Synergistic Hydrolysis of Carboxymethyl Cellulose and Acid-Swollen Cellulose by Two Endoglucanases (CelZ and CelY) from Erwinia chrysanthemi}, url = {http://dx.doi.org/10.1128/JB.182.20.5676-5682.2000}, volume = {182}, year = {2000} }

TY - JOUR ID - citeulike:3980208 L3 - citeulike-article-id:3980208 N2 - Erwinia chrysanthemi produces a battery of hydrolases and lyases which are very effective in the maceration of plant cell walls. Although two endoglucanases (CelZ and CelY; formerly EGZ and EGY) are produced, CelZ represents approximately 95% of the total carboxymethyl cellulase activity. In this study, we have examined the effectiveness of CelY and CelZ alone and of combinations of both enzymes using carboxymethyl cellulose (CMC) and amorphous cellulose (acid-swollen cellulose) as substrates. Synergy was observed with both substrates. Maximal synergy (1.8-fold) was observed for combinations containing primarily CelZ; the ratio of enzyme activities produced was similar to those produced by cultures of E. chrysanthemi. CelY and CelZ were quite different in substrate preference. CelY was unable to hydrolyze soluble cellooligosaccharides (cellotetraose and cellopentaose) but hydrolyzed CMC to fragments averaging 10.7 glucosyl units. In contrast, CelZ readily hydrolyzed cellotetraose, cellopentaose, and amorphous cellulose to produce cellobiose and cellotriose as dominant products. CelZ hydrolyzed CMC to fragments averaging 3.6 glucosyl units. In combination, CelZ and CelY hydrolyzed CMC to products averaging 2.3 glucosyl units. Synergy did not require the simultaneous presence of both enzymes. Enzymatic modification of the substrate by CelY increased the rate and extent of hydrolysis by CelZ. Full synergy was retained by the sequential hydrolysis of CMC, provided CelY was used as the first enzyme. A general mechanism is proposed to explain the synergy between these two enzymes based primarily on differences in substrate preference. 10.1128/JB.182.20.5676-5682.2000 IS - 20 JF - J. Bacteriol. EP - 5682 TI - Synergistic Hydrolysis of Carboxymethyl Cellulose and Acid-Swollen Cellulose by Two Endoglucanases (CelZ and CelY) from Erwinia chrysanthemi VL - 182 SP - 5676 KW - cellulase KW - cellulose KW - cmc KW - endoglucanase AU - Zhou, Shengde AU - Ingram, Lonnie PY - 2000/10/15/ UR - http://dx.doi.org/10.1128/JB.182.20.5676-5682.2000 ER -