Human Cathelicidin (LL-37), a Multifunctional Peptide, is Expressed by Ocular Surface Epithelia and has Potent Antibacterial and Antiviral Activity Export

@article{citeulike:6095855, abstract = {<i>Purpose</i>: This study determined whether LL-37 (cathelicidin) is expressed by conjunctival and corneal epithelia as part of ocular host defense. The antimicrobial activity of LL-37 was also assessed <i>in vitro</i> against <i>Pseudomonas aeruginosa</i> (PA), <i>Staphylococcus aureus</i> (SA), <i>Staphylococcus epidermidis</i> (SE), herpes simplex virus type 1 (HSV-1), and adenovirus (Ad). <i>Methods</i>: Expression of LL-37/hCAP 18 mRNA and LL-37 protein was determined by reverse transcription–polymerase chain reaction (RT-PCR) and immunoblotting, respectively, in scraped human corneal epithelium and primary cultured human corneal and conjunctival epithelial cells. The EC₅₀ values for three strains of PA and one each of SA and SE were determined for LL-37. LL-37 antiviral inhibition of HSV-1 and adenovirus was assessed by direct inactivation assays. Toxicity of LL-37 to A549 cells was evaluated by a MTT assay. <i>Results</i>: LL-37/hCAP18 mRNA and LL-37 peptide were expressed by human corneal and conjunctival epithelial cells. Antibacterial activity for LL-37 was demonstrated (EC₅₀ values for the three PA strains were 2.8 ± 1.3, 1.9 ± 0.3, and 3.6 ± 2.1; for SA: 1.6 ± 1.5; for SE: 1.3 ± 1.9 \&b.mu; g/ml). LL-37 produced a significant reduction (p \&lt; 0.001 ANOVA) in HSV-1 and Ad19 viral titers with distinctly different time-kill curves (p \&lt; 0.001). LL-37 (up to 111 \&b.mu; M) produced no toxicity in A549 cells. <i>Conclusions</i>: Corneal and conjunctival epithelia express LL-37 as part of mucosal innate immunity to protect against bacterial and viral ocular infections.}, author = {Gordon, Y. Jerold and Huang, Ling C. and Romanowski, Eric G. and Yates, Kathleen A. and Proske, Rita J. and McDermott, Alison M.}, citeulike-article-id = {6095855}, citeulike-linkout-0 = {http://dx.doi.org/10.1080/02713680590934111}, doi = {10.1080/02713680590934111}, journal = {Current Eye Research}, keywords = {adenovirus, cathelicidin, herpes\_simplex\_virus, hsv, innate\_immunity, ll-37, virus}, number = {5}, pages = {385--394}, posted-at = {2009-11-10 21:57:37}, priority = {3}, publisher = {Informa Healthcare}, title = {Human Cathelicidin (LL-37), a Multifunctional Peptide, is Expressed by Ocular Surface Epithelia and has Potent Antibacterial and Antiviral Activity}, url = {http://dx.doi.org/10.1080/02713680590934111}, volume = {30}, year = {2005} }

TY - JOUR ID - citeulike:6095855 L3 - citeulike-article-id:6095855 N2 - <i>Purpose</i>: This study determined whether LL-37 (cathelicidin) is expressed by conjunctival and corneal epithelia as part of ocular host defense. The antimicrobial activity of LL-37 was also assessed <i>in vitro</i> against <i>Pseudomonas aeruginosa</i> (PA), <i>Staphylococcus aureus</i> (SA), <i>Staphylococcus epidermidis</i> (SE), herpes simplex virus type 1 (HSV-1), and adenovirus (Ad). <i>Methods</i>: Expression of LL-37/hCAP 18 mRNA and LL-37 protein was determined by reverse transcription–polymerase chain reaction (RT-PCR) and immunoblotting, respectively, in scraped human corneal epithelium and primary cultured human corneal and conjunctival epithelial cells. The EC₅₀ values for three strains of PA and one each of SA and SE were determined for LL-37. LL-37 antiviral inhibition of HSV-1 and adenovirus was assessed by direct inactivation assays. Toxicity of LL-37 to A549 cells was evaluated by a MTT assay. <i>Results</i>: LL-37/hCAP18 mRNA and LL-37 peptide were expressed by human corneal and conjunctival epithelial cells. Antibacterial activity for LL-37 was demonstrated (EC₅₀ values for the three PA strains were 2.8 ± 1.3, 1.9 ± 0.3, and 3.6 ± 2.1; for SA: 1.6 ± 1.5; for SE: 1.3 ± 1.9 &b.mu; g/ml). LL-37 produced a significant reduction (p &lt; 0.001 ANOVA) in HSV-1 and Ad19 viral titers with distinctly different time-kill curves (p &lt; 0.001). LL-37 (up to 111 &b.mu; M) produced no toxicity in A549 cells. <i>Conclusions</i>: Corneal and conjunctival epithelia express LL-37 as part of mucosal innate immunity to protect against bacterial and viral ocular infections. IS - 5 JF - Current Eye Research EP - 394 TI - Human Cathelicidin (LL-37), a Multifunctional Peptide, is Expressed by Ocular Surface Epithelia and has Potent Antibacterial and Antiviral Activity PB - Informa Healthcare VL - 30 SP - 385 KW - adenovirus KW - cathelicidin KW - herpes_simplex_virus KW - hsv KW - innate_immunity KW - ll-37 KW - virus AU - Gordon, Jerold AU - Huang, Ling AU - Romanowski, Eric AU - Yates, Kathleen AU - Proske, Rita AU - McDermott, Alison PY - 2005/// UR - http://dx.doi.org/10.1080/02713680590934111 ER -