Habitat partitioning of denitrifying bacterial communities carrying nirS or nirK genes in the stratified water column of Lake Kinneret, Israel Export

@article{citeulike:3186992, abstract = {The community composition of denitrifying bacteria was studied in the stratified water column of Lake Kinneret. The nitrite reductase genes nirS and nirK were amplified by PCR from water samples taken at 1, 14, 19 and 22 m depth, which represent the epi-, meta- and hypolimnion of the lake. The PCR products were analyzed with terminal restriction fragment length polymorphism (T-RFLP) and clone libraries. The highest diversity of nirS denitrifying communities was observed at 1 m depth. According to the T-RFLP profiles and clone libraries of nirS products, 2 groups of denitrifiers were common to and dominant in all depths. Deduced protein sequences from one of these groups displayed low identity (77 \%) with other nirS sequences reported in GenBank. Denitrifying bacterial communities with nirK were most diverse at 22 m and showed highest similarity to those at 19 m depth. Sequences unrelated to nirK dominated the clone libraries from 1 m depth, suggesting that denitrifying bacteria with copper-containing nitrite reductase were less frequent at this depth. The results suggest that microorganisms with nirK and those with nirS respond differently to the environmental conditions in the stratified water column of Lake Kinneret.}, author = {Junier, Pilar and Kim, Ok-Sun and Witzel, Karl-Paul and Imhoff, Johannes F. and Hadas, Ora}, citeulike-article-id = {3186992}, citeulike-linkout-0 = {http://www.int-res.com/abstracts/ame/v51/n2/p129-140/}, journal = {Aquatic Microbial Ecology}, keywords = {denitrification, ecology, nirk, nirs}, number = {2}, pages = {129--140}, posted-at = {2008-09-03 16:05:58}, priority = {0}, title = {Habitat partitioning of denitrifying bacterial communities carrying nirS or nirK genes in the stratified water column of Lake Kinneret, Israel}, url = {http://www.int-res.com/abstracts/ame/v51/n2/p129-140/}, volume = {51}, year = {2008} }

TY - JOUR ID - citeulike:3186992 L3 - citeulike-article-id:3186992 N2 - The community composition of denitrifying bacteria was studied in the stratified water column of Lake Kinneret. The nitrite reductase genes nirS and nirK were amplified by PCR from water samples taken at 1, 14, 19 and 22 m depth, which represent the epi-, meta- and hypolimnion of the lake. The PCR products were analyzed with terminal restriction fragment length polymorphism (T-RFLP) and clone libraries. The highest diversity of nirS denitrifying communities was observed at 1 m depth. According to the T-RFLP profiles and clone libraries of nirS products, 2 groups of denitrifiers were common to and dominant in all depths. Deduced protein sequences from one of these groups displayed low identity (77 %) with other nirS sequences reported in GenBank. Denitrifying bacterial communities with nirK were most diverse at 22 m and showed highest similarity to those at 19 m depth. Sequences unrelated to nirK dominated the clone libraries from 1 m depth, suggesting that denitrifying bacteria with copper-containing nitrite reductase were less frequent at this depth. The results suggest that microorganisms with nirK and those with nirS respond differently to the environmental conditions in the stratified water column of Lake Kinneret. IS - 2 JF - Aquatic Microbial Ecology EP - 140 TI - Habitat partitioning of denitrifying bacterial communities carrying nirS or nirK genes in the stratified water column of Lake Kinneret, Israel VL - 51 SP - 129 KW - denitrification KW - ecology KW - nirk KW - nirs AU - Junier, Pilar AU - Kim, Ok-Sun AU - Witzel, Karl-Paul AU - Imhoff, Johannes AU - Hadas, Ora PY - 2008/// UR - http://www.int-res.com/abstracts/ame/v51/n2/p129-140/ ER -