Windshield splatter analysis with the Galaxy metagenomic pipeline Export

@article{citeulike:5938416, abstract = {10.1101/gr.094508.109 How many species inhabit our immediate surroundings? A straightforward collection technique suitable for answering this question is known to anyone who has ever driven a car at highway speeds. The windshield of a moving vehicle is subjected to numerous insect strikes and can be used as a collection device for representative sampling. Unfortunately the analysis of biological material collected in that manner, as with most metagenomic studies, proves to be rather demanding due to the large number of required tools and considerable computational infrastructure. In this study, we use organic matter collected by a moving vehicle to design and test a comprehensive pipeline for phylogenetic profiling of metagenomic samples that includes all steps from processing and quality control of data generated by next-generation sequencing technologies to statistical analyses and data visualization. To the best of our knowledge, this is also the first publication that features a live online supplement providing access to exact analyses and workflows used in the article.}, author = {Kosakovsky Pond, Sergei and Wadhawan, Samir and Chiaromonte, Francesca and Ananda, Guruprasad and Chung, Wen-Yu and Taylor, James and Nekrutenko, Anton}, citeulike-article-id = {5938416}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gr.094508.109}, citeulike-linkout-1 = {http://genome.cshlp.org/content/gr.094508.109v1/.abstract}, citeulike-linkout-2 = {http://genome.cshlp.org/content/gr.094508.109v1/.full.pdf}, citeulike-linkout-3 = {http://genome.cshlp.org/cgi/content/abstract/19/11/2144}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/19819906}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=19819906}, day = {9}, doi = {10.1101/gr.094508.109}, journal = {Genome Research}, keywords = {metagenome, methods}, month = {November}, number = {11}, pages = {2144--2153}, posted-at = {2009-11-09 14:50:43}, priority = {2}, title = {Windshield splatter analysis with the Galaxy metagenomic pipeline}, url = {http://dx.doi.org/10.1101/gr.094508.109}, volume = {19}, year = {2009} }

TY - JOUR ID - citeulike:5938416 L3 - citeulike-article-id:5938416 N2 - 10.1101/gr.094508.109 How many species inhabit our immediate surroundings? A straightforward collection technique suitable for answering this question is known to anyone who has ever driven a car at highway speeds. The windshield of a moving vehicle is subjected to numerous insect strikes and can be used as a collection device for representative sampling. Unfortunately the analysis of biological material collected in that manner, as with most metagenomic studies, proves to be rather demanding due to the large number of required tools and considerable computational infrastructure. In this study, we use organic matter collected by a moving vehicle to design and test a comprehensive pipeline for phylogenetic profiling of metagenomic samples that includes all steps from processing and quality control of data generated by next-generation sequencing technologies to statistical analyses and data visualization. To the best of our knowledge, this is also the first publication that features a live online supplement providing access to exact analyses and workflows used in the article. IS - 11 JF - Genome Research EP - 2153 TI - Windshield splatter analysis with the Galaxy metagenomic pipeline VL - 19 SP - 2144 KW - metagenome KW - methods AU - Kosakovsky Pond, Sergei AU - Wadhawan, Samir AU - Chiaromonte, Francesca AU - Ananda, Guruprasad AU - Chung, Wen-Yu AU - Taylor, James AU - Nekrutenko, Anton PY - 2009/11/09/ UR - http://dx.doi.org/10.1101/gr.094508.109 ER -