A System of Counteracting Feedback Loops Regulates Cdc42p Activity during Spontaneous Cell Polarization Export

@article{citeulike:3226085, abstract = {Summary Cellular polarization is often a response to distinct extracellular or intracellular cues, such as nutrient gradients or cortical landmarks. However, in the absence of such cues, some cells can still select a polarization axis at random. Positive feedback loops promoting localized activation of the GTPase Cdc42p are central to this process in budding yeast. Here, we explore spontaneous polarization during bud site selection in mutant yeast cells that lack functional landmarks. We find that these cells do not select a single random polarization axis, but continuously change this axis during the G1 phase of the cell cycle. This is reflected in traveling waves of activated Cdc42p which randomly explore the cell periphery. Our integrated computational and in vivo analyses of these waves reveal a negative feedback loop that competes with the aforementioned positive feedback loops to regulate Cdc42p activity and confer dynamic responsiveness on the robust initiation of cell polarization.}, author = {Ozbudak, Ertugrul M. and Becskei, Attila and van Oudenaarden, Alexander}, citeulike-article-id = {3226085}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.devcel.2005.08.014}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S1534580705003345}, citeulike-linkout-2 = {http://www.sciencedirect.com/science/article/B6WW3-4H7T3KK-H/2/f655429eeee7981f6fb106f2a45a0667}, doi = {10.1016/j.devcel.2005.08.014}, issn = {15345807}, journal = {Developmental Cell}, keywords = {activation, activity, cdc42, cerevisiae, construct, crib, domain, gfp, localization}, month = {October}, number = {4}, pages = {565--571}, posted-at = {2008-09-11 19:06:14}, priority = {2}, title = {A System of Counteracting Feedback Loops Regulates Cdc42p Activity during Spontaneous Cell Polarization}, url = {http://dx.doi.org/10.1016/j.devcel.2005.08.014}, volume = {9}, year = {2005} }

TY - JOUR ID - citeulike:3226085 L3 - citeulike-article-id:3226085 N2 - Summary Cellular polarization is often a response to distinct extracellular or intracellular cues, such as nutrient gradients or cortical landmarks. However, in the absence of such cues, some cells can still select a polarization axis at random. Positive feedback loops promoting localized activation of the GTPase Cdc42p are central to this process in budding yeast. Here, we explore spontaneous polarization during bud site selection in mutant yeast cells that lack functional landmarks. We find that these cells do not select a single random polarization axis, but continuously change this axis during the G1 phase of the cell cycle. This is reflected in traveling waves of activated Cdc42p which randomly explore the cell periphery. Our integrated computational and in vivo analyses of these waves reveal a negative feedback loop that competes with the aforementioned positive feedback loops to regulate Cdc42p activity and confer dynamic responsiveness on the robust initiation of cell polarization. IS - 4 JF - Developmental Cell SN - 15345807 EP - 571 TI - A System of Counteracting Feedback Loops Regulates Cdc42p Activity during Spontaneous Cell Polarization VL - 9 SP - 565 KW - activation KW - activity KW - cdc42 KW - cerevisiae KW - construct KW - crib KW - domain KW - gfp KW - localization AU - Ozbudak, Ertugrul AU - Becskei, Attila AU - van Oudenaarden, Alexander PY - 2005/10// UR - http://dx.doi.org/10.1016/j.devcel.2005.08.014 ER -