Mto2p, a Novel Fission Yeast Protein Required for Cytoplasmic Microtubule Organization and Anchoring of the Cytokinetic Actin Ring Export

@article{citeulike:3502586, abstract = {Microtubules regulate diverse cellular processes, including chromosome segregation, nuclear positioning, and cytokinesis. In many organisms, microtubule nucleation requires {gamma}-tubulin and associated proteins present at specific microtubule organizing centers (MTOCs). In fission yeast, interphase cytoplasmic microtubules originate from poorly characterized interphase MTOCs and spindle pole body (SPB), and during late anaphase from the equatorial MTOC (EMTOC). It has been previously shown that Mto1p (Mbo1p/Mod20p) function is important for the organization/nucleation of all cytoplasmic microtubules. Here, we show that Mto2p, a novel protein, interacts with Mto1p and is important for establishing a normal interphase cytoplasmic microtubule array. In addition, mto2{Delta} cells fail to establish a stable EMTOC and localize {gamma}-tubulin complex members to this medial structure. As predicted from these functions, Mto2p localizes to microtubules, the SPB, and the EMTOC in an Mto1p-dependent manner. mto2{Delta} cells fail to anchor the cytokinetic actin ring in the medial region of the cell and under conditions that mildly perturb actin structures, these rings unravel in mto2{Delta} cells. Our results suggest that the Mto2p and the EMTOC are critical for anchoring the cytokinetic actin ring to the medial region of the cell and for proper coordination of mitosis with cytokinesis. 10.1091/mbc.E04-12-1043}, author = {Venkatram, Srinivas and Jennings, Jennifer L. and Link, Andrew and Gould, Kathleen L.}, citeulike-article-id = {3502586}, citeulike-linkout-0 = {http://dx.doi.org/10.1091/mbc.E04-12-1043}, citeulike-linkout-1 = {http://www.molbiolcell.org/cgi/content/abstract/16/6/3052}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/15800064}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=15800064}, day = {1}, doi = {10.1091/mbc.E04-12-1043}, journal = {Mol. Biol. Cell}, keywords = {emtoc, imtoc, microtubules, mt, mtoc, organization}, month = {June}, number = {6}, pages = {3052--3063}, posted-at = {2008-11-11 05:47:18}, priority = {2}, title = {Mto2p, a Novel Fission Yeast Protein Required for Cytoplasmic Microtubule Organization and Anchoring of the Cytokinetic Actin Ring}, url = {http://dx.doi.org/10.1091/mbc.E04-12-1043}, volume = {16}, year = {2005} }

TY - JOUR ID - citeulike:3502586 L3 - citeulike-article-id:3502586 N2 - Microtubules regulate diverse cellular processes, including chromosome segregation, nuclear positioning, and cytokinesis. In many organisms, microtubule nucleation requires {gamma}-tubulin and associated proteins present at specific microtubule organizing centers (MTOCs). In fission yeast, interphase cytoplasmic microtubules originate from poorly characterized interphase MTOCs and spindle pole body (SPB), and during late anaphase from the equatorial MTOC (EMTOC). It has been previously shown that Mto1p (Mbo1p/Mod20p) function is important for the organization/nucleation of all cytoplasmic microtubules. Here, we show that Mto2p, a novel protein, interacts with Mto1p and is important for establishing a normal interphase cytoplasmic microtubule array. In addition, mto2{Delta} cells fail to establish a stable EMTOC and localize {gamma}-tubulin complex members to this medial structure. As predicted from these functions, Mto2p localizes to microtubules, the SPB, and the EMTOC in an Mto1p-dependent manner. mto2{Delta} cells fail to anchor the cytokinetic actin ring in the medial region of the cell and under conditions that mildly perturb actin structures, these rings unravel in mto2{Delta} cells. Our results suggest that the Mto2p and the EMTOC are critical for anchoring the cytokinetic actin ring to the medial region of the cell and for proper coordination of mitosis with cytokinesis. 10.1091/mbc.E04-12-1043 IS - 6 JF - Mol. Biol. Cell EP - 3063 TI - Mto2p, a Novel Fission Yeast Protein Required for Cytoplasmic Microtubule Organization and Anchoring of the Cytokinetic Actin Ring VL - 16 SP - 3052 KW - emtoc KW - imtoc KW - microtubules KW - mt KW - mtoc KW - organization AU - Venkatram, Srinivas AU - Jennings, Jennifer AU - Link, Andrew AU - Gould, Kathleen PY - 2005/06/01/ UR - http://dx.doi.org/10.1091/mbc.E04-12-1043 ER -