Polarized Growth in Budding Yeast in the Absence of a Localized Formin Export

@article{citeulike:4308239, abstract = {Monitoring Editor: David G. DrubinPolarity is achieved partly through the localized assembly of the cytoskeleton. During growth in budding yeast, the bud cortex and neck localized formins, Bni1p and Bnr1p, nucleate and assemble actin cables that extend along the bud-mother axis, providing tracks for secretory vesicle delivery. Localized formins are believed to determine the location and polarity of cables, hence growth. However, yeast expressing the nonlocalized actin nucleating/assembly FH1-FH2 domains of Bnr1p or Bni1p as the sole formin grow well. Although cables are significantly disorganized, analysis of directed transport of secretory vesicles is still biased toward the bud, reflecting a bias in correctly oriented cables, thereby permitting polarized growth. Myosin-II, localized at the bud neck, contributes to polarized growth as a mutant unable to interact with F-actin further compromises growth in cells with an unlocalized formin, but not with a localized one. Our results show that multiple mechanisms contribute to cable orientation and polarized growth, with localized formins and myosin-II being two major contributors. 10.1091/mbc.E09-03-0194}, author = {Gao, Lina and Bretscher, Anthony}, citeulike-article-id = {4308239}, citeulike-linkout-0 = {http://dx.doi.org/10.1091/mbc.E09-03-0194}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19297522}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19297522}, day = {18}, doi = {10.1091/mbc.E09-03-0194}, journal = {Mol. Biol. Cell}, keywords = {absence, actin, budding, cerevisiae, deletion, formin, myo2, polarization, polarized, yeast}, month = {March}, pages = {E09-03-0194+}, posted-at = {2009-04-14 18:19:09}, priority = {2}, title = {Polarized Growth in Budding Yeast in the Absence of a Localized Formin}, url = {http://dx.doi.org/10.1091/mbc.E09-03-0194}, year = {2009} }

TY - JOUR ID - citeulike:4308239 L3 - citeulike-article-id:4308239 N2 - Monitoring Editor: David G. DrubinPolarity is achieved partly through the localized assembly of the cytoskeleton. During growth in budding yeast, the bud cortex and neck localized formins, Bni1p and Bnr1p, nucleate and assemble actin cables that extend along the bud-mother axis, providing tracks for secretory vesicle delivery. Localized formins are believed to determine the location and polarity of cables, hence growth. However, yeast expressing the nonlocalized actin nucleating/assembly FH1-FH2 domains of Bnr1p or Bni1p as the sole formin grow well. Although cables are significantly disorganized, analysis of directed transport of secretory vesicles is still biased toward the bud, reflecting a bias in correctly oriented cables, thereby permitting polarized growth. Myosin-II, localized at the bud neck, contributes to polarized growth as a mutant unable to interact with F-actin further compromises growth in cells with an unlocalized formin, but not with a localized one. Our results show that multiple mechanisms contribute to cable orientation and polarized growth, with localized formins and myosin-II being two major contributors. 10.1091/mbc.E09-03-0194 JF - Mol. Biol. Cell TI - Polarized Growth in Budding Yeast in the Absence of a Localized Formin SP - E09-03-0194 KW - absence KW - actin KW - budding KW - cerevisiae KW - deletion KW - formin KW - myo2 KW - polarization KW - polarized KW - yeast AU - Gao, Lina AU - Bretscher, Anthony PY - 2009/03/18/ UR - http://dx.doi.org/10.1091/mbc.E09-03-0194 ER -