Systematic Deletion Analysis of Fission Yeast Protein Kinases Export

@article{citeulike:6095800, abstract = {Eukaryotic protein kinases are key molecules mediating signal transduction that play a pivotal role in the regulation of various biological processes, including cell cycle progression, cellular morphogenesis, development, and cellular response to environmental changes. A total of 106 eukaryotic protein kinase catalytic-domain-containing proteins have been found in the entire fission yeast genome, 44\% (or 64\%) of which possess orthologues (or nearest homologues) in humans, based on sequence similarity within catalytic domains. Systematic deletion analysis of all putative protein kinase-encoding genes have revealed that 17 out of 106 were essential for viability, including three previously uncharacterized putative protein kinases. Although the remaining 89 protein kinase mutants were able to form colonies under optimal growth conditions, 46\% of the mutants exhibited hypersensitivity to at least 1 of the 17 different stress factors tested. Phenotypic assessment of these mutants allowed us to arrange kinases into functional groups. Based on the results of this assay, we propose also the existence of four major signaling pathways that are involved in the response to 17 stresses tested. Microarray analysis demonstrated a significant correlation between the expression signature and growth phenotype of kinase mutants tested. Our complete microarray data sets are available at http://giscompute.gis.a-star.edu.sg/[\~{}]gisljh/kinome. 10.1128/EC.4.4.799-813.2005}, author = {Bimbo, Andrea and Jia, Yonghui and Poh, Siew L. and Karuturi and den Elzen, Nicole and Peng, Xu and Zheng, Liling and O'Connell, Matthew and Liu, Edison T. and Balasubramanian, Mohan K. and Liu, Jianhua}, citeulike-article-id = {6095800}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/EC.4.4.799-813.2005}, citeulike-linkout-1 = {http://ec.asm.org/content/4/4/799.abstract}, citeulike-linkout-2 = {http://ec.asm.org/content/4/4/799.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/15821139}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=15821139}, day = {1}, doi = {10.1128/EC.4.4.799-813.2005}, journal = {Eukaryotic Cell}, keywords = {deletion, kinase, library}, month = {April}, number = {4}, pages = {799--813}, posted-at = {2009-11-10 20:59:17}, priority = {2}, title = {Systematic Deletion Analysis of Fission Yeast Protein Kinases}, url = {http://dx.doi.org/10.1128/EC.4.4.799-813.2005}, volume = {4}, year = {2005} }

TY - JOUR ID - citeulike:6095800 L3 - citeulike-article-id:6095800 N2 - Eukaryotic protein kinases are key molecules mediating signal transduction that play a pivotal role in the regulation of various biological processes, including cell cycle progression, cellular morphogenesis, development, and cellular response to environmental changes. A total of 106 eukaryotic protein kinase catalytic-domain-containing proteins have been found in the entire fission yeast genome, 44% (or 64%) of which possess orthologues (or nearest homologues) in humans, based on sequence similarity within catalytic domains. Systematic deletion analysis of all putative protein kinase-encoding genes have revealed that 17 out of 106 were essential for viability, including three previously uncharacterized putative protein kinases. Although the remaining 89 protein kinase mutants were able to form colonies under optimal growth conditions, 46% of the mutants exhibited hypersensitivity to at least 1 of the 17 different stress factors tested. Phenotypic assessment of these mutants allowed us to arrange kinases into functional groups. Based on the results of this assay, we propose also the existence of four major signaling pathways that are involved in the response to 17 stresses tested. Microarray analysis demonstrated a significant correlation between the expression signature and growth phenotype of kinase mutants tested. Our complete microarray data sets are available at http://giscompute.gis.a-star.edu.sg/[~]gisljh/kinome. 10.1128/EC.4.4.799-813.2005 IS - 4 JF - Eukaryotic Cell EP - 813 TI - Systematic Deletion Analysis of Fission Yeast Protein Kinases VL - 4 SP - 799 KW - deletion KW - kinase KW - library AU - Bimbo, Andrea AU - Jia, Yonghui AU - Poh, Siew AU - Karuturi AU - den Elzen, Nicole AU - Peng, Xu AU - Zheng, Liling AU - O'Connell, Matthew AU - Liu, Edison AU - Balasubramanian, Mohan AU - Liu, Jianhua PY - 2005/04/01/ UR - http://dx.doi.org/10.1128/EC.4.4.799-813.2005 ER -