Development of High-Performance Liquid Chromatography–Time-of-Flight Mass Spectrometry for the Simultaneous Characterization and Quantitative Analysis of Gingerol-Related Compounds in Ginger Products

Liquid chromatography?time-of-flight mass spectrometry (LC-TOF/MS) was established for the simultaneous separation, identification, and quantification of gingerol-related compounds in ginger products. The established method has been shown to provide a satisfactory linearity (r > 0.999) in a wide range (5?5000 ng/mL), low limits of detection and quantification, high precision, and inter- and intraday repeatability. The detection sensitivity of gingerols and shogaols by TOF/MS was 70?100 times higher than conventional UV detection at 288 nm. In this study, 19 ginerol-related compounds in the samples were identified and quantified by the established LC-TOF/MS method. The dried ginger powder products contained the highest quantity of gingerol-related compounds (7126.3?13789.0 ?g/g), followed by fresh ginger products (2007.9?2790.0 ?g/g), powdered ginger tea products (77.29?81.75 ?g/g), and hot water ginger extracts (54.59?123.23 ?g/mL). Shogaols were not found in fresh gingers. This paper represents the first report on the LC-TOF/MS analysis for the simultaneous characterization and quantification of gingerol-related compounds in ginger products.