Chromosomal instability mediated by non-B DNA: cruciform conformation and not DNA sequence is responsible for recurrent translocation in humans. Export

@article{citeulike:5659877, abstract = {Chromosomal aberrations have been thought to be random events. However, recent findings introduce a new paradigm in which certain DNA segments have the potential to adopt unusual conformations that lead to genomic instability and nonrandom chromosomal rearrangement. One of the best-studied examples is the palindromic AT-rich repeat (PATRR), which induces recurrent constitutional translocations in humans. Here, we established a plasmid-based model that promotes frequent intermolecular rearrangements between two PATRRs in HEK293 cells. In this model system, the proportion of PATRR plasmid that extrudes a cruciform structure correlates to the levels of rearrangement. Our data suggest that PATRR-mediated translocations are attributable to unusual DNA conformations that confer a common pathway for chromosomal rearrangements in humans.}, author = {Inagaki, Hidehito and Ohye, Tamae and Kogo, Hiroshi and Kato, Takema and Bolor, Hasbaira and Taniguchi, Mariko and Shaikh, Tamim H. and Emanuel, Beverly S. and Kurahashi, Hiroki}, citeulike-article-id = {5659877}, citeulike-linkout-0 = {http://dx.doi.org/10.1101/gr.079244.108}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18997000}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18997000}, doi = {10.1101/gr.079244.108}, issn = {1088-9051}, journal = {Genome research}, keywords = {at-rich, autosomal, cruciform, meiosis, palindromes, palindromic, patrr, rearrangements, translocation}, month = {February}, number = {2}, pages = {191--198}, posted-at = {2009-08-27 21:39:17}, priority = {0}, title = {Chromosomal instability mediated by non-B DNA: cruciform conformation and not DNA sequence is responsible for recurrent translocation in humans.}, url = {http://dx.doi.org/10.1101/gr.079244.108}, volume = {19}, year = {2009} }

TY - JOUR ID - citeulike:5659877 L3 - citeulike-article-id:5659877 N2 - Chromosomal aberrations have been thought to be random events. However, recent findings introduce a new paradigm in which certain DNA segments have the potential to adopt unusual conformations that lead to genomic instability and nonrandom chromosomal rearrangement. One of the best-studied examples is the palindromic AT-rich repeat (PATRR), which induces recurrent constitutional translocations in humans. Here, we established a plasmid-based model that promotes frequent intermolecular rearrangements between two PATRRs in HEK293 cells. In this model system, the proportion of PATRR plasmid that extrudes a cruciform structure correlates to the levels of rearrangement. Our data suggest that PATRR-mediated translocations are attributable to unusual DNA conformations that confer a common pathway for chromosomal rearrangements in humans. IS - 2 JF - Genome research SN - 1088-9051 EP - 198 TI - Chromosomal instability mediated by non-B DNA: cruciform conformation and not DNA sequence is responsible for recurrent translocation in humans. VL - 19 SP - 191 KW - at-rich KW - autosomal KW - cruciform KW - meiosis KW - palindromes KW - palindromic KW - patrr KW - rearrangements KW - translocation AU - Inagaki, Hidehito AU - Ohye, Tamae AU - Kogo, Hiroshi AU - Kato, Takema AU - Bolor, Hasbaira AU - Taniguchi, Mariko AU - Shaikh, Tamim AU - Emanuel, Beverly AU - Kurahashi, Hiroki PY - 2009/02// UR - http://dx.doi.org/10.1101/gr.079244.108 ER -