Functional characterization of three G protein-coupled receptors for pigment dispersing factors in caenorhabditis elegans. Export

@article{citeulike:3337151, abstract = {Here, we report the identification, cloning and functional characterization of three Caenorhabditis elegans G protein-coupled Pigment Dispersing Factor (PDF) receptors, which we designated as Ce\_PDFR-1a, b and c. They represent three splice isoforms of the same gene (C13B9.4) which share a high degree of similarity with the Drosophila PDF receptor and are distantly related to the mammalian vasoactive intestinal peptide receptors (VPAC2) and calcitonin receptors. In a reverse pharmacological screen, three bioactive C. elegans neuropeptides, which were recently identified as the Drosophila PDF orthologues, were able to activate these receptors in a dose-dependent manner with nanomolar potency (isoforms a and b). Integrated GFP reporter constructs reveal the expression of these PDF receptors in all body wall muscle cells and many head and tail neurons involved in the integration of environmental stimuli and the control of locomotion. Using a custom data analysis system, we demonstrate the involvement of this newly discovered neuropeptide signaling system in the regulation of locomotor behavior. Overexpression of PDF-2 phenocopies the locomotor defects of a PDF-1 null mutant, suggesting that they elicit opposite effects on locomotion through the identified PDF receptors. Our findings strengthen the hypothesis that the PDF signaling system, that imposes the circadian clock rhythm on behavior in Drosophila, has been functionally conserved throughout the protostomian evolutionary lineage.}, author = {Janssen, T. and Husson, S. J. and Lindemans, M. and Mertens, I. and Rademakers, S. and Ver and Geysen, J. and Jansen, G. and Schoofs, L.}, citeulike-article-id = {3337151}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M709060200}, citeulike-linkout-1 = {http://www.jbc.org/cgi/content/abstract/283/22/15241?view=long&pmid=18390545}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18390545}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18390545}, citeulike-linkout-4 = {http://www.wormbase.org/db/misc/paper?name=WBPaper00031656}, doi = {10.1074/jbc.M709060200}, journal = {J Biol Chem}, keywords = {c, dispersing, elegans, factors, gpcr, pigment}, posted-at = {2009-06-19 14:38:07}, priority = {0}, title = {Functional characterization of three G protein-coupled receptors for pigment dispersing factors in caenorhabditis elegans.}, url = {http://dx.doi.org/10.1074/jbc.M709060200}, year = {2008} }

TY - JOUR ID - citeulike:3337151 L3 - citeulike-article-id:3337151 N2 - Here, we report the identification, cloning and functional characterization of three Caenorhabditis elegans G protein-coupled Pigment Dispersing Factor (PDF) receptors, which we designated as Ce_PDFR-1a, b and c. They represent three splice isoforms of the same gene (C13B9.4) which share a high degree of similarity with the Drosophila PDF receptor and are distantly related to the mammalian vasoactive intestinal peptide receptors (VPAC2) and calcitonin receptors. In a reverse pharmacological screen, three bioactive C. elegans neuropeptides, which were recently identified as the Drosophila PDF orthologues, were able to activate these receptors in a dose-dependent manner with nanomolar potency (isoforms a and b). Integrated GFP reporter constructs reveal the expression of these PDF receptors in all body wall muscle cells and many head and tail neurons involved in the integration of environmental stimuli and the control of locomotion. Using a custom data analysis system, we demonstrate the involvement of this newly discovered neuropeptide signaling system in the regulation of locomotor behavior. Overexpression of PDF-2 phenocopies the locomotor defects of a PDF-1 null mutant, suggesting that they elicit opposite effects on locomotion through the identified PDF receptors. Our findings strengthen the hypothesis that the PDF signaling system, that imposes the circadian clock rhythm on behavior in Drosophila, has been functionally conserved throughout the protostomian evolutionary lineage. JF - J Biol Chem TI - Functional characterization of three G protein-coupled receptors for pigment dispersing factors in caenorhabditis elegans. KW - c KW - dispersing KW - elegans KW - factors KW - gpcr KW - pigment AU - Janssen, T AU - Husson, SJ AU - Lindemans, M AU - Mertens, I AU - Rademakers, S AU - Ver AU - Geysen, J AU - Jansen, G AU - Schoofs, L PY - 2008/// UR - http://dx.doi.org/10.1074/jbc.M709060200 ER -