Recombinant aequorin as a reporter for receptor-mediated changes of intracellular Ca2+-levels in Drosophila S2 cells Export

@article{citeulike:4921099, abstract = {Abstract.\ \  The bioluminescent Ca2+-sensitive reporter protein, aequorin, was employed to develop an insect cell-based functional assay system for monitoring receptor-mediated changes of intracellular Ca2 +-concentrations. Drosophila Schneider\ 2 (S2) cells were genetically engineered to stably express both apoaequorin and the insect tachykinin-related peptide receptor, STKR. Lom-TK\ III, an STKR agonist, was shown to elicit concentration-dependent bioluminescent responses in these S2-STKR-Aeq cells. The EC50 value for the calcium effect detected by means of aequorin appeared to be nearly identical to the one that was measured by means of Fura-2, a fluorescent Ca2 +-indicator. In addition, this aequorin-based method was also utilised to study receptor antagonists. Experimental analysis of the effects exerted by spantide\ I, II and III, three potent substance\ P antagonists, on Lom-TK\ III-stimulated S2-STKR-Aeq cells showed that these compounds antagonise STKR-mediated responses in a concentration-dependent manner. The rank order of inhibitory potencies was spantide\ III \> spantide\ II \> spantide\ I.}, author = {Torfs, Herbert and Poels, Jeroen and Detheux, Michel and Dupriez, Vincent and Van Loy, Tom and Vercammen, Linda and Vassart, Gilbert and Parmentier, Marc and Vanden}, citeulike-article-id = {4921099}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s10158-001-0013-2}, citeulike-linkout-1 = {http://www.springerlink.com/content/kkmkxb2jw9hnb9wt}, day = {25}, doi = {10.1007/s10158-001-0013-2}, journal = {Invertebrate Neuroscience}, keywords = {aequorin, calcium, cell, drosophile, s2}, month = {April}, number = {3}, pages = {119--124}, posted-at = {2009-06-22 09:11:29}, priority = {0}, title = {Recombinant aequorin as a reporter for receptor-mediated changes of intracellular Ca2+-levels in Drosophila S2 cells}, url = {http://dx.doi.org/10.1007/s10158-001-0013-2}, volume = {4}, year = {2002} }

TY - JOUR ID - citeulike:4921099 L3 - citeulike-article-id:4921099 N2 - Abstract.   The bioluminescent Ca2+-sensitive reporter protein, aequorin, was employed to develop an insect cell-based functional assay system for monitoring receptor-mediated changes of intracellular Ca2 +-concentrations. Drosophila Schneider 2 (S2) cells were genetically engineered to stably express both apoaequorin and the insect tachykinin-related peptide receptor, STKR. Lom-TK III, an STKR agonist, was shown to elicit concentration-dependent bioluminescent responses in these S2-STKR-Aeq cells. The EC50 value for the calcium effect detected by means of aequorin appeared to be nearly identical to the one that was measured by means of Fura-2, a fluorescent Ca2 +-indicator. In addition, this aequorin-based method was also utilised to study receptor antagonists. Experimental analysis of the effects exerted by spantide I, II and III, three potent substance P antagonists, on Lom-TK III-stimulated S2-STKR-Aeq cells showed that these compounds antagonise STKR-mediated responses in a concentration-dependent manner. The rank order of inhibitory potencies was spantide III > spantide II > spantide I. IS - 3 JF - Invertebrate Neuroscience EP - 124 TI - Recombinant aequorin as a reporter for receptor-mediated changes of intracellular Ca2+-levels in Drosophila S2 cells VL - 4 SP - 119 KW - aequorin KW - calcium KW - cell KW - drosophile KW - s2 AU - Torfs, Herbert AU - Poels, Jeroen AU - Detheux, Michel AU - Dupriez, Vincent AU - Van Loy, Tom AU - Vercammen, Linda AU - Vassart, Gilbert AU - Parmentier, Marc AU - Vanden PY - 2002/04/25/ UR - http://dx.doi.org/10.1007/s10158-001-0013-2 ER -