Serotonin receptor cDNA cloned from Lymnaea stagnalis. Export

@article{citeulike:5435599, abstract = {Serotonin (5-HT) is a major neurotransmitter that influences various behaviors, neuronal plasticity, learning, and memory in molluscs. Although the physiology of 5-HT transmission in molluscs is well studied, little is known about the pharmacology and diversity of the 5-HT receptor system. Based on the high homology of genes coding for guanine nucleotide-binding protein (G protein)-coupled receptors, we have cloned a gene for the Lymnaea stagnalis 5-HT (5HTlym) receptor. The putative receptor protein, 509 amino acids long, has highest homology with the Drosophila 5-HT receptors and mammalian 5HT1 receptors. As revealed by RNA blot-hybridization analysis, two mRNA species of 2.3 and 3.2 kb are detected in the central nervous system of Lymnaea. Transient expression of 5HTlym in COS-7 cells showed saturable [3H]lysergic acid diethylamide binding with an estimated dissociation constant of 0.9 nM. The 5HTlym receptor exhibited a mixed 5HT-like pharmacology that cannot be precisely categorized with existing mammalian classification nomenclature. However, the 5HTlym receptor does display some characteristics that have been attributed to the putative mammalian vascular 5HT1-like receptor.}, author = {Sugamori, K. S. and Sunahara, R. K. and Guan, H. C. and Bulloch, A. G. and Tensen, C. P. and Seeman, P. and Niznik, H. B. and Van Tol, H. H.}, citeulike-article-id = {5435599}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/8093556}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=8093556}, day = {1}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {lymne, receptor, serotonin}, month = {January}, number = {1}, pages = {11--15}, posted-at = {2009-08-27 13:02:56}, priority = {2}, title = {Serotonin receptor cDNA cloned from Lymnaea stagnalis.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/8093556}, volume = {90}, year = {1993} }

TY - JOUR ID - citeulike:5435599 L3 - citeulike-article-id:5435599 N2 - Serotonin (5-HT) is a major neurotransmitter that influences various behaviors, neuronal plasticity, learning, and memory in molluscs. Although the physiology of 5-HT transmission in molluscs is well studied, little is known about the pharmacology and diversity of the 5-HT receptor system. Based on the high homology of genes coding for guanine nucleotide-binding protein (G protein)-coupled receptors, we have cloned a gene for the Lymnaea stagnalis 5-HT (5HTlym) receptor. The putative receptor protein, 509 amino acids long, has highest homology with the Drosophila 5-HT receptors and mammalian 5HT1 receptors. As revealed by RNA blot-hybridization analysis, two mRNA species of 2.3 and 3.2 kb are detected in the central nervous system of Lymnaea. Transient expression of 5HTlym in COS-7 cells showed saturable [3H]lysergic acid diethylamide binding with an estimated dissociation constant of 0.9 nM. The 5HTlym receptor exhibited a mixed 5HT-like pharmacology that cannot be precisely categorized with existing mammalian classification nomenclature. However, the 5HTlym receptor does display some characteristics that have been attributed to the putative mammalian vascular 5HT1-like receptor. IS - 1 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 EP - 15 TI - Serotonin receptor cDNA cloned from Lymnaea stagnalis. VL - 90 SP - 11 KW - lymne KW - receptor KW - serotonin AU - Sugamori, KS AU - Sunahara, RK AU - Guan, HC AU - Bulloch, AG AU - Tensen, CP AU - Seeman, P AU - Niznik, HB AU - Van Tol, HH PY - 1993/01/01/ UR - http://view.ncbi.nlm.nih.gov/pubmed/8093556 ER -