The Lymnaea Cardioexcitatory Peptide (LyCEP) Receptor: A G-Protein-Coupled Receptor for a Novel Member of the RFamide Neuropeptide Family Export

@article{citeulike:5656617, abstract = {A novel G-protein-coupled receptor (GRL106) resembling neuropeptide Y and tachykinin receptors was cloned from the mollusc Lymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL106 activated a calcium-dependent chloride channel. Using this response as a bioassay, we purified the ligand for GRL106, Lymnaea cardioexcitatory peptide (LyCEP), an RFamide-type decapeptide (TPHWRPQGRF-NH2) displaying significant similarity to the Achatina cardioexcitatory peptide (ACEP-1) as well as to the recently identified family of mammalian prolactin-releasing peptides. In the Lymnaea brain, the cells that produce egg-laying hormone are the predominant site of GRL106 gene expression and appear to be innervated by LyCEP-containing fibers. Indeed, LyCEP application transiently hyperpolarizes isolated egg-laying hormone cells. In the Lymnaea pericardium, LyCEP-containing fibers end blindly at the pericardial lumen, and the heart is stimulated by LyCEP in vitro. These data confirm that LyCEP is an RFamide ligand for GRL106.}, author = {Tensen, Cornelis P. and Cox, Kingsley J. . A. and Smit, August B. and van der Schors, Roel C. and Meyerhof, Wolfgang and Richter, Dietmar and Planta, Rudi J. and Hermann, Petra M. and van Minnen, Jan and Geraerts, Wijnand P. . M. and Knol, Jaco C. and Burke, Julian F. and Vreugdenhil, Erno and van Heerikhuizen, Harm}, citeulike-article-id = {5656617}, citeulike-linkout-0 = {http://www.jneurosci.org/cgi/content/abstract/18/23/9812}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/9822740}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=9822740}, day = {1}, journal = {J. Neurosci.}, keywords = {cardioexcitatory, lymne, peptide, receptor}, month = {December}, number = {23}, pages = {9812--9821}, posted-at = {2009-08-27 12:45:37}, priority = {2}, title = {The Lymnaea Cardioexcitatory Peptide (LyCEP) Receptor: A G-Protein-Coupled Receptor for a Novel Member of the RFamide Neuropeptide Family}, url = {http://www.jneurosci.org/cgi/content/abstract/18/23/9812}, volume = {18}, year = {1998} }

TY - JOUR ID - citeulike:5656617 L3 - citeulike-article-id:5656617 N2 - A novel G-protein-coupled receptor (GRL106) resembling neuropeptide Y and tachykinin receptors was cloned from the mollusc Lymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL106 activated a calcium-dependent chloride channel. Using this response as a bioassay, we purified the ligand for GRL106, Lymnaea cardioexcitatory peptide (LyCEP), an RFamide-type decapeptide (TPHWRPQGRF-NH2) displaying significant similarity to the Achatina cardioexcitatory peptide (ACEP-1) as well as to the recently identified family of mammalian prolactin-releasing peptides. In the Lymnaea brain, the cells that produce egg-laying hormone are the predominant site of GRL106 gene expression and appear to be innervated by LyCEP-containing fibers. Indeed, LyCEP application transiently hyperpolarizes isolated egg-laying hormone cells. In the Lymnaea pericardium, LyCEP-containing fibers end blindly at the pericardial lumen, and the heart is stimulated by LyCEP in vitro. These data confirm that LyCEP is an RFamide ligand for GRL106. IS - 23 JF - J. Neurosci. EP - 9821 TI - The Lymnaea Cardioexcitatory Peptide (LyCEP) Receptor: A G-Protein-Coupled Receptor for a Novel Member of the RFamide Neuropeptide Family VL - 18 SP - 9812 KW - cardioexcitatory KW - lymne KW - peptide KW - receptor AU - Tensen, Cornelis AU - Cox, Kingsley AU - Smit, August AU - van der Schors, Roel AU - Meyerhof, Wolfgang AU - Richter, Dietmar AU - Planta, Rudi AU - Hermann, Petra AU - van Minnen, Jan AU - Geraerts, Wijnand AU - Knol, Jaco AU - Burke, Julian AU - Vreugdenhil, Erno AU - van Heerikhuizen, Harm PY - 1998/12/01/ UR - http://www.jneurosci.org/cgi/content/abstract/18/23/9812 ER -