<b>Molecular cloning and characterization of an invertebrate homologue of a neuropeptide Y receptor</b> Export

@article{citeulike:5656631, abstract = {Neuropeptide Y is an abundant and physiologically important peptide in vertebrates having effects on food intake, sexual behaviour, blood pressure and circadian rhythms. Neuropeptide Y homologues have been found in invertebrates, where they are very likely to play similar, important roles. Although five neuropeptide Y2010receptor subtypes have been identified in mammals, none has been reported from invertebrates. Here we describe the cloning of a neuropeptide Y2010receptor from the brain of the snail Lymnaea stagnalis. The identity of the receptor was deduced by expressing the neuropeptide Y2010receptor2010encoding cDNA in Chinese Hamster Ovary cells, which were subsequently challenged with size2010fractionated Lymnaea brain extracts. An active peptide, selected on the basis of its ability to induce changes in cAMP levels, was purified to homogeneity, analysed by mass spectrometry and amino acid sequence determination, and turned out to be a Lymnaea homologue of neuropeptide Y.}, address = {; Department of Biochemistry and Molecular Biology, Graduate School of Neurosciences, Research Institute Neurosciences, ; Department of Molecular Neurobiology, Graduate School of Neurosciences, Research Institute Neurosciences, De Boelelaan 1087, NL1081 HV Amsterdam, the Netherlands ; Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK ; Department of Pharmacochemistry, LeidenAmsterdam Center for Drug Research, De Boelelaan 1083, NL1081 HV Amsterdam, the Netherlands}, author = {Tensen, Cornelis P. and Cox, Kingsley J. A. and Burke, Julian F. and Leurs, Rob and van der Schors, Roel C. and Geraerts, Wijnand P. M. and Vreugdenhil, Erno and van Heerikhuizen1, Harm}, citeulike-article-id = {5656631}, citeulike-linkout-0 = {http://dx.doi.org/10.1046/j.1460-9568.1998.00350.x}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/119128473/ABSTRACT}, doi = {10.1046/j.1460-9568.1998.00350.x}, issn = {1460-9568}, journal = {European Journal of Neuroscience}, keywords = {lymne, neuropeptide, receptor, y}, number = {11}, pages = {3409--3416}, posted-at = {2009-08-27 12:48:35}, priority = {2}, title = {<b>Molecular cloning and characterization of an invertebrate homologue of a neuropeptide Y receptor</b>}, url = {http://dx.doi.org/10.1046/j.1460-9568.1998.00350.x}, volume = {10}, year = {1998} }

TY - JOUR ID - citeulike:5656631 L3 - citeulike-article-id:5656631 N2 - Neuropeptide Y is an abundant and physiologically important peptide in vertebrates having effects on food intake, sexual behaviour, blood pressure and circadian rhythms. Neuropeptide Y homologues have been found in invertebrates, where they are very likely to play similar, important roles. Although five neuropeptide Y2010receptor subtypes have been identified in mammals, none has been reported from invertebrates. Here we describe the cloning of a neuropeptide Y2010receptor from the brain of the snail Lymnaea stagnalis. The identity of the receptor was deduced by expressing the neuropeptide Y2010receptor2010encoding cDNA in Chinese Hamster Ovary cells, which were subsequently challenged with size2010fractionated Lymnaea brain extracts. An active peptide, selected on the basis of its ability to induce changes in cAMP levels, was purified to homogeneity, analysed by mass spectrometry and amino acid sequence determination, and turned out to be a Lymnaea homologue of neuropeptide Y. IS - 11 JF - European Journal of Neuroscience SN - 1460-9568 AD - ; Department of Biochemistry and Molecular Biology, Graduate School of Neurosciences, Research Institute Neurosciences, ; Department of Molecular Neurobiology, Graduate School of Neurosciences, Research Institute Neurosciences, De Boelelaan 1087, NL1081 HV Amsterdam, the Netherlands ; Biochemistry Laboratory, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK ; Department of Pharmacochemistry, LeidenAmsterdam Center for Drug Research, De Boelelaan 1083, NL1081 HV Amsterdam, the Netherlands EP - 3416 TI - <b>Molecular cloning and characterization of an invertebrate homologue of a neuropeptide Y receptor</b> VL - 10 SP - 3409 KW - lymne KW - neuropeptide KW - receptor KW - y AU - Tensen, Cornelis AU - Cox, Kingsley AU - Burke, Julian AU - Leurs, Rob AU - van der Schors, Roel AU - Geraerts, Wijnand AU - Vreugdenhil, Erno AU - van Heerikhuizen1, Harm PY - 1998/// UR - http://dx.doi.org/10.1046/j.1460-9568.1998.00350.x ER -