DNA identification of skeletal remains from the World War II mass graves uncovered in Slovenia. Export

@article{citeulike:1835643, abstract = {AIM: To present the joint effort of three institutions in the identification of human remains from the World War II found in two mass graves in the area of Skofja Loka, Slovenia. METHODS: The remains of 27 individuals were found in two small and closely located mass graves. The DNA was isolated from bone and teeth samples using either standard phenol/chloroform alcohol extraction or optimized Qiagen DNA extraction procedure. Some recovered samples required the employment of additional DNA purification methods, such as N-buthanol treatment. Quantifiler Human DNA Quantification Kit was used for DNA quantification. PowerPlex 16 kit was used to simultaneously amplify 15 short tandem repeat (STR) loci. Matching probabilities were estimated using the DNA View program. RESULTS: Out of all processed samples, 15 remains were fully profiled at all 15 STR loci. The other 12 profiles were partial. The least successful profile included 13 loci. Also, 69 referent samples (buccal swabs) from potential living relatives were collected and profiled. Comparison of victims' profile against referent samples database resulted in 4 strong matches. In addition, 5 other profiles were matched to certain referent samples with lower probability. CONCLUSION: Our results show that more than 6 decades after the end of the World War II, DNA analysis may significantly contribute to the identification of the remains from that period. Additional analysis of Y-STRs and mitochondrial DNA (mtDNA) markers will be performed in the second phase of the identification project.}, address = {Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina. marjanovd@hotmail.com}, author = {Marjanovi\'{c}, Damir and Durmi\'{c}-Pasi\'{c}, Adaleta and Bakal, Narcisa and Haveri\'{c}, Sanin and Kalamuji\'{c}, Belma and Kovacevi\'{c}, Lejla and Rami\'{c}, Jasmin and Pojski\'{c}, Naris and Skaro, Vedrana and Proji\'{c}, Petar and Bajrovi\'{c}, Kasim and Hadziselimovi\'{c}, Rifat and Drobnic, Katja and Huffine, Edwin and Davoren, Jon and Primorac, Dragan}, citeulike-article-id = {1835643}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/17696306}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=17696306}, citeulike-linkout-2 = {http://www.cmj.hr/2007/48/4/17696306.htm}, issn = {1332-8166}, journal = {Croatian medical journal}, keywords = {disaster, dna, id, war}, month = {August}, number = {4}, pages = {513--519}, posted-at = {2009-02-03 11:59:20}, priority = {5}, title = {DNA identification of skeletal remains from the World War II mass graves uncovered in Slovenia.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/17696306}, volume = {48}, year = {2007} }

TY - JOUR ID - citeulike:1835643 L3 - citeulike-article-id:1835643 N2 - AIM: To present the joint effort of three institutions in the identification of human remains from the World War II found in two mass graves in the area of Skofja Loka, Slovenia. METHODS: The remains of 27 individuals were found in two small and closely located mass graves. The DNA was isolated from bone and teeth samples using either standard phenol/chloroform alcohol extraction or optimized Qiagen DNA extraction procedure. Some recovered samples required the employment of additional DNA purification methods, such as N-buthanol treatment. Quantifiler Human DNA Quantification Kit was used for DNA quantification. PowerPlex 16 kit was used to simultaneously amplify 15 short tandem repeat (STR) loci. Matching probabilities were estimated using the DNA View program. RESULTS: Out of all processed samples, 15 remains were fully profiled at all 15 STR loci. The other 12 profiles were partial. The least successful profile included 13 loci. Also, 69 referent samples (buccal swabs) from potential living relatives were collected and profiled. Comparison of victims' profile against referent samples database resulted in 4 strong matches. In addition, 5 other profiles were matched to certain referent samples with lower probability. CONCLUSION: Our results show that more than 6 decades after the end of the World War II, DNA analysis may significantly contribute to the identification of the remains from that period. Additional analysis of Y-STRs and mitochondrial DNA (mtDNA) markers will be performed in the second phase of the identification project. IS - 4 JF - Croatian medical journal SN - 1332-8166 AD - Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina. marjanovd@hotmail.com EP - 519 TI - DNA identification of skeletal remains from the World War II mass graves uncovered in Slovenia. VL - 48 SP - 513 KW - disaster KW - dna KW - id KW - war AU - Marjanović, Damir AU - Durmić-Pasić, Adaleta AU - Bakal, Narcisa AU - Haverić, Sanin AU - Kalamujić, Belma AU - Kovacević, Lejla AU - Ramić, Jasmin AU - Pojskić, Naris AU - Skaro, Vedrana AU - Projić, Petar AU - Bajrović, Kasim AU - Hadziselimović, Rifat AU - Drobnic, Katja AU - Huffine, Edwin AU - Davoren, Jon AU - Primorac, Dragan PY - 2007/08// UR - http://view.ncbi.nlm.nih.gov/pubmed/17696306 ER -