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Journal of Molecular Biology, Vol. 230, No. 4. (April 1993), pp. 1317-1319, doi:10.1006/jmbi.1993.1248 Key: citeulike:11934789
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The major transitions of the eukaryotic cell cycle are triggered by cyclin-dependent protein kinases. We report the purification and crystallization of the catalytic subunit of human cyclin-dependent kinase 2 (CDK2), which has been implicated in the control of the G1/S transition. CDK2 was purified in large quantities from insect cells infected with a recombinant baculovirus, and crystals of the protein were prepared and subjected to preliminary X-ray diffraction analysis.
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