A novel germ-cell-specific protein, SHIP1, forms a complex with chromatin remodeling activity during spermatogenesis. Export

@article{citeulike:3505689, abstract = {To determine the mechanisms of spermatogenesis, it is essential to identify and characterize germ-cell-specific genes. Here, we describe a protein encoded by a novel germ-cell-specific gene, Mm.290718/ZFP541, identified from the mouse spermatocyte UniGene library. The protein contains specific motifs and domains potentially involved in DNA binding and chromatin reorganization. An antibody against Mm.290718/ZFP541 revealed the existence of the protein in testicular spermatogenic cells (159 kDa), but not testicular and mature sperm. Immunostaining analysis of cells at various stages of spermatogenesis consistently showed that the protein is present in spermatocytes and round spermatids only. Transfection assays and immunofluorescence studies indicate that the protein is localized specifically in the nucleus. Proteomic analyses performed to explore the functional characteristics of Mm.290718/ZFP541 showed that the protein forms a unique complex. Other major components of the complex included histone deacetylase 1 (HDAC1) and heat-shock protein A2. Disappearance of Mm.290718/ZFP541 was highly correlated with hyperacetylation in spermatids during spermatogenesis, and specific domains of the protein were involved in the regulation of interactions and nuclear localization of HDAC1. Further, we found that premature hyperacetylation, induced by an HDAC inhibitor, is associated with an alteration in the integrity of Mm.290718/ZFP541 in spermatogenic cells. Our results collectively suggest that the Mm.290718/ZFP541 complex is implicated in chromatin remodeling during spermatogenesis, and provide further information on the previously unknown molecular mechanism. Consequently, we re-designate Mm.290718/ZFP541 as 'SHIP1' representing 'spermatogenic cell HDAC-interacting protein 1'.}, author = {Choi, Eunyoung and Han, Cecil and Park, Inju and Lee, Boyeon and Jin, Sora and Choi, Heejin and Kim, Do Han H. and Park, Zee Yong Y. and Eddy, Edward M M. and Cho, Chunghee}, citeulike-article-id = {3505689}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M805590200}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18849567}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18849567}, day = {10}, doi = {10.1074/jbc.M805590200}, issn = {0021-9258}, journal = {The Journal of biological chemistry}, keywords = {chromatin, epigenetic, germcell, mouse}, month = {October}, posted-at = {2008-11-12 12:08:52}, priority = {2}, title = {A novel germ-cell-specific protein, SHIP1, forms a complex with chromatin remodeling activity during spermatogenesis.}, url = {http://dx.doi.org/10.1074/jbc.M805590200}, year = {2008} }

TY - JOUR ID - citeulike:3505689 L3 - citeulike-article-id:3505689 N2 - To determine the mechanisms of spermatogenesis, it is essential to identify and characterize germ-cell-specific genes. Here, we describe a protein encoded by a novel germ-cell-specific gene, Mm.290718/ZFP541, identified from the mouse spermatocyte UniGene library. The protein contains specific motifs and domains potentially involved in DNA binding and chromatin reorganization. An antibody against Mm.290718/ZFP541 revealed the existence of the protein in testicular spermatogenic cells (159 kDa), but not testicular and mature sperm. Immunostaining analysis of cells at various stages of spermatogenesis consistently showed that the protein is present in spermatocytes and round spermatids only. Transfection assays and immunofluorescence studies indicate that the protein is localized specifically in the nucleus. Proteomic analyses performed to explore the functional characteristics of Mm.290718/ZFP541 showed that the protein forms a unique complex. Other major components of the complex included histone deacetylase 1 (HDAC1) and heat-shock protein A2. Disappearance of Mm.290718/ZFP541 was highly correlated with hyperacetylation in spermatids during spermatogenesis, and specific domains of the protein were involved in the regulation of interactions and nuclear localization of HDAC1. Further, we found that premature hyperacetylation, induced by an HDAC inhibitor, is associated with an alteration in the integrity of Mm.290718/ZFP541 in spermatogenic cells. Our results collectively suggest that the Mm.290718/ZFP541 complex is implicated in chromatin remodeling during spermatogenesis, and provide further information on the previously unknown molecular mechanism. Consequently, we re-designate Mm.290718/ZFP541 as 'SHIP1' representing 'spermatogenic cell HDAC-interacting protein 1'. JF - The Journal of biological chemistry SN - 0021-9258 TI - A novel germ-cell-specific protein, SHIP1, forms a complex with chromatin remodeling activity during spermatogenesis. KW - chromatin KW - epigenetic KW - germcell KW - mouse AU - Choi, Eunyoung AU - Han, Cecil AU - Park, Inju AU - Lee, Boyeon AU - Jin, Sora AU - Choi, Heejin AU - Kim, Do Han AU - Park, Zee Yong AU - Eddy, Edward M AU - Cho, Chunghee PY - 2008/10/10/ UR - http://dx.doi.org/10.1074/jbc.M805590200 ER -